Pseudotime Ordering Single-Cell Transcriptomic ofβCells Pancreatic Islets in Health and Type 2 Diabetes

作     者：Kaixuan Bao Zhicheng Cui Hui Wang Hui Xiao Ting Li Xingxing Kong Tiemin Liu 

作者机构：Human Phenome InstituteFudan University825 Zhangheng RoadShanghai 201203China State Key Laboratory of Genetic EngineeringSchool of Life Sciencesand Collaborative Innovation Center for Genetics and DevelopmentFudan UniversityShanghai 200438China Shanghai Key Laboratory of Metabolic Remodeling and HealthInstitute of Metabolism and Integrative BiologyFudan UniversityShanghai 200433China Department of Endocrinology and MetabolismZhongshan HospitalFudan UniversityShanghai 200032China 

出 版 物：《Phenomics》 (表型组学（英文）)

年 卷 期：2021年第1卷第5期

页      面：199-210页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2019YFA0801900,2018YFA0800300) the National Natural Science Foundation of China(31971074) the Science and Technology Innovation Action Plan of Shanghai Science and Technology Committee(18140901300) the Open Research Fund of the National key laboratory of genetic engineering(SKLGE1803) the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01) Shanghai Frontiers Science Research Base of Exercise and Metabolic Health 

主　　题：Single-cell RNA seq Islets βcell Pseudotime T2D 

摘      要：βcells are defined by the ability to produce and secret *** studies have evaluated that human pancreaticβcells are heterogeneous and demonstrated the transcript alterations ofβcell subpopulation in ***-cell RNA sequence(scRNA-seq)analysis helps us to refine the cell types signatures and understand the role of theβcells during metabolic challenges and ***,we construct the pseudotime trajectory ofβcells from publicly available scRNA-seq data in health and type 2 diabetes(T2D)based on highly dispersed and highly expressed genes using *** identified three major states including 1)Normal branch,2)Obesity-like branch and 3)T2D-like branch based on biomarker genes and genes that give rise to bifurcation in the trajectory.βcell function-maintain-related genes,insulin expression-related genes,and T2D-related genes enriched in three branches,*** pseudotime spectrum might suggest thatβcells transition among different *** application of pseudotime analysis is conducted to clarify the different cell states,providing novel insights into the pathology ofβcells in T2D.