High-quality reference genome and annotation aids understanding of berry development for evergreen blueberry(Vaccinium darrowii)
作者机构：Genome Science and Technology ProgramUniversity of TennesseeKnoxvilleTN 37996USA USDA-ARS Gen omics and Bioi nformatics Research Un itRaleighNCUSA Department of Crop and Soil SciencesNorth Carolina State UniversityRaleighNCUSA USDA-ARS Thad Cochran Southern Horticultural LaboratoryPoplarvilleMSUSA Department of Entomology and Plant PathologyUniversity of TennesseeKnoxvilleTN 37996USA
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：3321-3336页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学]
摘 要：Vaccinium darrowii Camp(2 n=2×=24)is a native North American blueberry species and an important source oftraits such as low chill requirement in commercial southern highbush blueberry breeding(Vaccinium coymbosum,2n=4 ×=48).We present a chromosomal-scale genome of *** generated by the combination of PacBio sequencing and high throughput chromatin conformation capture(Hi-C)scaffolding technologies,yielding a total length of 1.06 Gigabases(Gb).Over 97.8%of the genome sequences are scaffolded into 24 chromosomes representing the two *** primary haplotype assembly of V,darrowii contains 34,809 protein-coding *** to a *** haplotype assembly reveals high collinearity between the two genomes with small intrachromosomal rearrangements in eight chromosome *** small RNA sequencing,the annotation was further expanded to include more than 200,000 small RNA loci and 638 microRNAs expressed in berry *** analysis across fruit development stages indicates that genes involved in photosynthesis are downregulated,while genes involved in flavonoid and anthocyanin biosynthesis are signi ficantly increased at the late stage of berry ripening.A high-quality reference genome and accompanying annotation of *** is a signi ficant new resource for assessing the evergreen blueberry contribution to the breeding of southern highbush blueberries.
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