Over expression of resistin in adipose tissue of the obese induces insulin resistance

作     者：Sadashiv Sunita Tiwari S Dhananjai Bhola N Paul Sandeep Kumar Abhijit Chandra Mahendra PS Negi 

作者机构：Department of Physiology CSM Medical UniversityLucknow 226003 India Immunobiology Division Indian Institute of Toxicological ResearchLucknow 226001 India Department of Surgery CSM Medical UniversityLucknow 226003 India Department of Gastroenterology CSM Medical UniversityLucknow 226003 India Institute for Data Computing and TrainingLucknow 226021 India 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2012年第3卷第7期

页      面：135-141页

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：Supported by Indian Council of Medical Research, New Delhi,India Central Council Research in Yoga and Naturopathy,New Delhi, India, to Sadashiv and Tiwari S 

主　　题：Resistin Subcutaneous adipose tissue Insu-lin resistance Obesity Body mass index 

摘      要：AIM: To compare resistin mRNA expression in subcutaneous adipose tissue (SAT) and its correlation with insulin resistance (IR) in postmenopausal obese women. METHODS: A total of 68 postmenopausal women (non obese = 34 and obese = 34) were enrolled for the study. The women of the two groups were age matched (49-70 years). Fasting blood samples were collected at admission and abdominal SAT was obtained during surgery for gall bladder stones or hysterectomy. Physical parameters [age, height, weight, body mass index (BMI)] were measured. Biochemical (plasma insulin and plasma glucose) parameters were estimated by enzymatic methods. RNA was isolated by the Trizol method.SAT resistin mRNA expression was done by real time- reverse transcription polymerase chain reaction (RT-PCR) by using Quanti Tect SYBR Green RT-PCR master mix. Data was analyzed using independent Student s t test, correlation and simple linear regression analysis. RESULTS: The mean weight (52.81 ± 8.04 kg vs 79.56 ± 9.91 kg; P 0.05). CONCLUSION: Increased SAT resistin mRNA expres-sion probably leads to inducing insulin resistance and thus may be associated with obesity-related disorders in postmenopausal obese women.