Effects of DHRS3 in C2C12 Myoblast Differentiation and Mouse Skeletal Muscle Injury

作     者：Zhang Wen-yu Xu Jia-hui Zhang Chun-yu Tong Hui-li Li Shu-feng Yan Yun-qin Zhang Wen-yu;Xu Jia-hui;Zhang Chun-yu;Tong Hui-li;Li Shu-feng;Yan Yun-qin

作者机构：College of Life Sciences and TechnologyNortheast Agricultural UniversityHarbin 150030China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2021年第28卷第3期

页      面：38-47页

学科分类：0710[理学-生物学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071009[理学-细胞生物学] 100101[医学-人体解剖与组织胚胎学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 10[医学] 

基　　金：Supported by the Natural Science Foundation of Heilongjiang Province(C2017025) 

主　　题：DHRS3 C2C12 cell differentiation mouse skeletal muscle injury 

摘      要：Myoblast differentiation is an essential process during skeletal muscle development.C2C12 myoblast is a commonly used experimental model to study muscle cell differentiation in ***/reductase(SDR family)member 3(DHRS3)is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of *** experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts ***,the effect of DHRS3 on mouse muscle cell differentiation was *** objective of current study was to determine if DHRS3 affected muscle cell differentiation,and if DHRS3 was involved in muscle *** expression was determined by western blot and immunofluorescence *** activation and inhibition of DHRS3 increased and decreased C2C12 myoblast differentiation respectively,which indicated that DHRS3 could affect C2C12 myoblast ***3 expression was significantly changed during muscle regeneration,with the regeneration of muscle injury,the expression of DHRS3 tended to increase first and then *** suggested that DHRS3 might be involved in muscle *** summary,this study confirmed the involvement of DHRS3 in C2C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development.