C_(17)-fengycin B,produced by deep-sea-derived Bacillus subtilis,possessing a strong antifungal activity against Fusarium solani

作     者：Weixiang LIU Chaomin SUN Weixiang LIU;Chaomin SUN

作者机构：CAS and Shandong Province Key Laboratory of Experimental Marine BiologyInstitute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and BiotechnologyPilot NationalLaboratory for Marine Science and Technology(Qingdao)Qingdao 266237China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2021年第39卷第5期

页      面：1938-1947页

核心收录：

学科分类：0710[理学-生物学] 090403[农学-农药学(可授农学、理学学位）] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 0815[工学-水利工程] 0904[农学-植物保护] 

基　　金：the National Key R&D Program of China(No.2018YFC0310800) the China Ocean Mineral Resources R&D Association Grant(No.DY135-B2-14) the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA22050301) the Taishan Young Scholar Program of Shandong Province(No.tsqn20161051) the Qingdao Innovation Leadership Program(No.18-1-2-7-zhc)for Chaomin SUN the China Postdoctoral Science Foundation(No.2019M652492)。 

主　　题：Bacillus species lipopeptide fengycin antifungal pesticide 

摘      要：Root rot disease caused by Fusarium solani is the most devastating disease of the tomato and legume crops in China.The metabolites of Bacillus species can inhibit many fungal diseases.In this study,the metabolites of deep-sea-derived bacterium Bacillus subtilis 2 H11 can significantly inhibit the growth of F.solani.The metabolite C_(17)-fengycin B,one of the cyclic lipopeptides,was identified by the combination of silica column chromatography,high-performance liquid chromatography(HPLC),high-energy collision induced dissociation mass spectrometry(HCD-MS)and tandem mass spectrometry(HCD-MS/MS).The results of scanning electron microscopy(SEM)and transmission electron microscopy(TEM)showed that C_(17)-fengycin B could destroy the structure of the hyphae and spores of F.solani.The antifungal activities of C_(17)-fengycin B against F.solani were tested at concentrations ranging from 0.05 mg/mL to 0.20 mg/mL.The results indicated that C_(17)-fengycin B inhibited the growth of F.solani with antifungal index of 89.80%at 0.20 mg/mL,and the antifungal activity of C_(17)-fengycin B was further verified by the pot experiment.In addition,the cytotoxicity experiment showed that C_(17)-fengycin B had good biocompatibility and was a potential candidate for the development of biocontrol pesticide in the future.