Diverse transcriptional patterns of homoeologous recombinant transcripts in triploid fish(Cyprinidae)

作     者：Li Ren Xueyin Zhang Jiaming Li Xiaojing Yan Xin Gao Jialin Cui Chenchen Tang Shaojun Liu Li Ren;Xueyin Zhang;Jiaming Li;Xiaojing Yan;Xin Gao;Jialin Cui;Chenchen Tang;Shaojun Liu

作者机构：State Key Laboratory of Developmental Biology of Freshwater FishHunan Normal UniversityChangsha 410081China College of Life SciencesHunan Normal UniversityChangsha 410081China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2021年第64卷第9期

页      面：1491-1501页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0908[农学-水产] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31702334,31730098,U19A2040 and31430088) the Key Research and Development Program of Hunan Province(2018NK2072 and 2017NK1031) the Earmarked Fund for China Agriculture Research System(CARS-45) 111 Project(D20007) High-Level Talent Agglomeration Program of Hunan China(2019RS1044) the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486)。 

主　　题：homoeologous recombination recombinant transcripts triploid innate immunity 

摘      要：Homoeologous recombination(HR),the exchange of homoeologous chromosomes,contributes to subgenome adaptation to diverse environments by producing various phenotypes.However,the potential relevance of HR and innate immunity is rarely described in triploid cyprinid fish species.In our study,two allotriploid genotypes(R_(2)C and RC_(2)),whose innate immunity was stronger than their inbred parents(Carassius auratus red var.and Cyprinus carpio L.),were obtained from backcrossing between male allotetraploids of C.auratus red var.×C.carpio L.and females of their two inbred parents,respectively.The work detected 140 HRs shared between the two triploids at the genomic level.Further,transcriptions of 54 homoeologous recombinant genes(HRGs)in R_(2)C and 65 HRGs in RC_(2) were detected using both Illumina and PacBio data.Finally,by comparing expressed recombinant reads to total expressed reads in each of the genes,a range of 0.1%-10% was observed in most of the 99-193 HRGs,of which six recombinant genes were classified asresponse to stimulus.These results not only provide a novel way to predict HRs in allopolyploids based on cross prediction at both genomic and transcriptional levels,but also insight into the potential relationship between HRs related to innate immunity and adaptation of the triploids and allotetraploids.