Effects of varying dietary folic acid during weaning stress of piglets

作     者：Lei Wang Xian Tan Huiru Wang Qiye Wang Pengfei Huang Yali Li Jianzhong Li Jing Huang Huansheng Yang Yulong Yin Lei Wang;Xian Tan;Huiru Wang;Qiye Wang;Pengfei Huang;Yali Li;Jianzhong Li;Jing Huang;Huansheng Yang;Yulong Yin

作者机构：Laboratory of Animal Nutrition and Human HealthHunan International Joint Laboratory of Animal Intestinal Ecology and HealthCollege of Life ScienceHunan Normal UniversityChangsha410081China Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionKey Laboratory of Agro-ecological Processes in Subtropical RegionScientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-CentralMinistry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China Department of NeuroscienceHengyang School of MedicineUniversity of South ChinaHengyang421000China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第1期

页      面：101-110页

核心收录：

学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：Key Programs of frontier scientific research of the Chinese Academy of Sciences(Grant No.QYZDY-SSW-SMC008) National Key R&D Program(Grant No.2016YFD0501201) Natural Science Foundation of Hunan Province(Grant No.2017JJ1020). 

主　　题：Folic acid Growth performance Antibiotic-free Intestinal morphology Post-weaning piglet 

摘      要：The present study was conducted to evaluate the effect of dietary folic acid on the growth performance,intestinal morphology,and intestinal epithelial cells renewal in post-weaning piglets.Twenty-eight piglets(weaned at day 21,initial body weight of 6.73±0.62 kg)were randomly allotted to 4 treat-ments with 7 pens per diet and 1 piglet per pen.The piglets were fed the same antibiotic-free and zinc oxide-free basal diets supplemented with folic acid at 0,3,9,and 18 mg/kg for 14 days.The results showed that dietary supplementation with folic acid increased villus height(VH)(P=0.003;linear,P=0.001),VH-to-crypt depth(VH:CD)ratio(P=0.002;linear,P=0.001),villus surface area(VSA)(P=0.026;linear,P=0.010).The analyzed parameters ADG,serum urea nitrogen(BUN)content,VH,VSA,and serum folate(SF)concentration responded linearly to the dietary folic acid concentration when the dietary folic acid concentration was below 4.42,5.26,4.79,3.47,and 3.53 mg/kg respectively(R2=0.995,0.995,0.999,0.999,0.872,P=0.09,0.07,0.09,0.09,0.36,respectively),as assessed by a two-linear broken-line regression.Above these breakpoints,the response of ADG,VH,VSA,and SF plateaued in response to changes in dietary folic acid concentration.Moreover,dietary supplementation with folic acid significantly increased the lactase(P=0.001;linear,P=0.001)and sucrase activities(P=0.021;linear,P=0.010)in the jejunal mucosa of weaned piglets.The mRNA expression of solute carrier family 6 member 19(SLC6a19),solute carrier family 1 member 1(SLC7a1),tumor necrosis factor-α(TNF-α),the number of Ki67 positive cells,and cell shedding rate had a significant linear contrast(P=0.023,0.021,0.038,0.049,and 0.008,respectively)in dietary folic acid groups.In conclusion,our results indicate that folic acid supplementation can improve the growth performance and intestinal morphology of weaned piglets by maintaining the balance of epithelial cell renewal.