Bautype: Capsule and Lipopolysaccharide Serotype Prediction for Acinetobacter baumannii Genome

作     者：Xiaoting Hua Qian Liang Li Fang Jintao He Meixia Wang Wenjie Hong Sebastian Leptihn Hengliang Wang Yunsong Yu Huan Chen 

作者机构：Department of Infectious DiseasesSir Run Run Shaw HospitalCollege of MedicineZhejiang UniversityHangzhouChina Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang ProvinceZhejiang Institute of MicrobiologyHangzhou 310012China NMPA Key laboratory for Testing and Risk Warning of Pharmaceutical MicrobiologyZhejiang Institute of MicrobiologyHangzhou 310012China Zhejiang University-University of Edinburgh InstituteSchool of MedicineZhejiang UniversityHangzhouChina State Key Laboratory of Pathogens and BiosecurityBeijing Institute of BiotechnologyBeijingChina. 

出 版 物：《Infectious Microbes & Diseases》 (感染微生物与疾病（英文）)

年 卷 期：2020年第2卷第1期

页      面：18-25页

学科分类：0710[理学-生物学] 100208[医学-临床检验诊断学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

基　　金：supported by the grants from the National Natural Science Foundation of China(31970128,31770142,81861138054,31670135) the Zhejiang Province Medical Platform(2020RC075). 

主　　题：serotype LOS CPS Acinetobacter WGS 

摘      要：Acinetobacter baumannii has three clusters of genes for polysaccharide synthesis.Capsular polysaccharides and lipopolysaccharides are major virulence determinants in many Gram-negative bacteria,and play key roles in the overall fitness and virulence in A.baumannii,meanwhile poly-beta-1-6-N-acetylglucosamine was critical for biofilm formation.A variety of studies have been conducted which focused on the surveillance of antimicrobial resistance genes and clones in A.baumannii.However,a tool to perform serotype prediction in A.baumannii is still lacking.Here we present Bautype,a tool for whole genome sequencingbased K and O locus typing in A.baumannii.Using Bautype,users can upload and analyze the genome assembled in a web browser,with the results being displayed in a tabular or graphical format.We demonstrated the high accuracy of predicting K and O locus diversity using Bautype when analyzing more than 3000 A.baumannii genomes.Using our software,we were able to analyze K and O locus diversity among 593 genomes belonging to ST208(3 K and 8 O).These diverse loci can serve as epidemiological markers for Multi-drug Resistance(MDR)strain subtyping.We also identified 45 novel O locus types.Our findings highlight the importance in monitoring the capsule type and LOS of A.baumannii strains for which Bautype is a tailored clinical software.Bautype is available at http://***.