Histological age estimation of the eggs of Calliphora vicina Robineau Desvoidy (Diptera: Calliphoridae)

作     者：Michael Pais Melanie S.Archer Michael Pais;Melanie S.Archer

作者机构：Department of Forensic MedicineVictorian Institute of Forensic MedicineMonash UniversitySouthbankVictoriaAustralia 

出 版 物：《Forensic Sciences Research》 (法庭科学研究（英文）)

年 卷 期：2018年第3卷第1期

页      面：40-51页

学科分类：0402[教育学-心理学(可授教育学、理学学位）] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0303[法学-社会学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0703[理学-化学] 0702[理学-物理学] 

基　　金：Victorian Institute of Forensic Medicine (VIFM) 

主　　题：Forensic science forensic entomology Calliphoridae Calliphora vicina eggs histology embryology 

摘      要：Aging blow fly eggs can be critical to a forensic investigation,but there are currently no forensically useful timelines describing internal anatomical changes in embryological development.This is partly due to the lack of an economical,rapid and technically simple histological technique to allow mass production of slides for research and casework.We present a histological method that uses a slightly modified standard laboratory processing run with 1 h fixation in 10% formalin,2 h softening in Molliflex and Haemotoxylin and Eosin (H&E) staining.We also present a summary of the internal anatomical changes that can be visualized using our technique in the developing eggs of Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae).We examined eggs from at least three different females grown at 15 ℃ and sampled at 6 h intervals,and eggs grown at 20 ℃ and sampled at 3 h intervals.Blind aging trials demonstrated that it is possible to accurately age material grown at 20 ℃ to within 6 h (but attempts to further narrow this interval resulted in errors in one-third of cases).It was also possible to see sufficient anatomical detail to age eggs preserved for forensic casework 5,8,9,10 and 11 years previously.Additionally,we determined that section quality was improved by 5 s fixation in hot water prior to preservation in ethanol.However,hot water fixation for longer than this increased the level of section artefact.