Nanobody-based immunosensing methods for safeguarding public health

作     者：Jiarong Zhang Hui Sun Wei Pei Huijun Jiang Jin Chen Jiarong Zhang;Hui Sun;Wei Pei;Huijun Jiang;Jin Chen

作者机构：Center for Global HealthThe Key Laboratory of Modern ToxicologyMinistry of EducationSchool of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China School of Chemistry and Chemical EngineeringSoutheast UniversityNanjingJiangsu 211189China School of PharmacyNanjing Medical UniversityNanjingJiangsu 211166China Jiangsu Province Engineering Research Center of Antibody DrugKey Laboratory of Antibody Technique of National Health CommissionNanjing Medical UniversityNanjingJiangsu 211166China. 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2021年第35卷第4期

页      面：318-326页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 080202[工学-机械电子工程] 08[工学] 0802[工学-机械工程] 10[医学] 

基　　金：the National Natural Science Foundation of China(No.U1703118) Natural Science Foundation of Jiangsu Higher Education Institutions of China(No.19KJA310003) Natural Science Foundation of Jiangsu Province(No.BK20181364) 

主　　题：immunosensing nanobody biomarker environmental pollutant food contamination 

摘      要：Immunosensing methods are biosensing techniques based on specific recognition of an antigen-antibody immunocomplex,which have become commonly used in safeguarding public *** advantage of antibody-related biotechnological advances,the utilization of an antigen-binding fragment of a heavy-chain-only antibody termed as nanobody holds significant biomedical *** with the conventional full-length antibody,a single-domain nanobody retaining cognate antigen specificity possesses remarkable physicochemical stability and structural adaptability,which enables a flexible and efficient molecular design of the immunosensing *** minireview aims to summarize the recent progress in immunosensing methods using nanobody targeting tumor markers,environmental pollutants,and foodborne microbes.