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Identification of two QTLs associated with high fruit acidity in apple using pooled genome sequencing analysis

作     者:Seunghyun Ban Kenong Xu Seunghyun Ban;Kenong Xu

作者机构:Horticulture SectionSchool of Integrative Plant ScienceCornell AgritechCornell UniversityGenevaNY 14456USA 

出 版 物:《Horticulture Research》 (园艺研究(英文))

年 卷 期:2020年第7卷第1期

页      面:560-573页

核心收录:

学科分类:0710[理学-生物学] 0831[工学-生物医学工程(可授工学、理学、医学学位)] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0836[工学-生物工程] 090201[农学-果树学] 0834[工学-风景园林学(可授工学、农学学位)] 

基  金:supported in part by the Agriculture and Food Research Initiative competitive grant no.2014-67013-21660 National Institute of Food and Agriculture US Department of Agriculture(NIFA USDA). 

主  题:fir acidity analysis 

摘      要:Acidity is a critical component determining apple fruit quality.Previous studies reported two major acidity quantitative trait loci(QTLs)on linkage groups(LGs)16(Ma)and 8(Ma3),respectively,and their homozygous genotypes mama and ma3ma3 usually confer low titratable acidity(TA)(10mgml^(−1))acidity levels.To date,the genetic control for high-acidity apples remains essentially unknown.In order to map QTLs associated with high acidity,two genomic DNA pools,one for high acidity and the other for regular acidity,were created in an interspecific F1 population Royal Gala(Malus domestica)×PI 613988(M.sieversii)of 191 fruit-bearing progenies.By Illumina paired-end sequencing of the high and regular acidity pools,1,261,640 single-nucleotide variants(SNVs)commonly present in both pools were detected.Using allele frequency directional difference and density(AFDDD)mapping approach,one region on chromosome 4 and another on chromosome 6 were identified to be putatively associated with high acidity,and were named Ma6 and Ma4,respectively.Trait association analysis of DNA markers independently developed from the Ma6 and Ma4 regions confirmed the mapping of Ma6 and Ma4.In the background of MaMa,20.6%of acidity variation could be explained by Ma6,28.5%by Ma4,and 50.7%by the combination of both.The effects of Ma6 and Ma4 in the background of Mama were also significant,but lower.These findings provide important genetic insight into high acidity in apple.

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