Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies

作     者：Sourav Das Leader Langbang Mahabul Haque Vinay Kumar Belwal Kripamoy Aguan Atanu Singha Roy Sourav Das;Leader Langbang;Mahabul Haque;Vinay Kumar Belwal;Kripamoy Aguan;Atanu Singha Roy

作者机构：Department of ChemistryNational Institute of Technology MeghalayaShillong793003India Department of Biotechnology&BioinformaticsNorth-Eastern Hill UniversityShillong793022India Department of Bioscience and BioengineeringIndian Institute of Technology GuwahatiAssam781039India 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2021年第11卷第4期

页      面：422-434页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 0817[工学-化学工程与技术] 08[工学] 0703[理学-化学] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：the Science and Engineering Research Board(ECR File No.ECR/2016/000159 and CRG File No.CRG/2019/000852) Government of India for funding this work 

主　　题：Green tea Polyphenols Silver nanoparticles Anti-bacterial Cytotoxicity Hen egg white lysozyme(HEWL) 

摘      要：Green synthesis of silver nanoparticles(AgNPs)has garnered tremendous interest as conventional methods include the use and production of toxic chemicals,products,by-products and *** this regard,the synthesis of AgNPs using green tea(GT)extract and two of its components,(-)-epigallocatechin gallate(EGCG)and(+)-catechin(Ct)as capping/stabilizing agents,is *** synthesized AgNPs showed antibacterial activity against the bacterial strains Staphylococcus aureus and Escherichia coli,along with anticancer activity against HeLa *** administering nanoparticles to the body,they come in contact with proteins and results in the formation of a protein corona;hence we studied the interactions of these biocompatible AgNPs with hen egg white lysozyme(HEWL)as a carrier *** quenching mechanism was accountable for the quenching of HEWL fluorescence by the *** binding constant(Kb)was found to be higher for EGCG-AgNPs((2.309±0.018)×104 M-1)than for GT-AgNPs and Ct-AgNPs towards ***-AgNPs increased the polarity near the binding site while Ct-AgNPs caused the opposite effect,but GT-AgNPs had no such observable *** dichroism studies indicated that the AgNPs had no such appreciable impact on the secondary structure of *** key findings of this research included the synthesis of AgNPs using GT extract and its constituent polyphenols,and showed significant antibacterial,anticancer and protein-binding ***-OH groups of the polyphenols drive the in situ capping/stabilization of the AgNPs during synthesis,which might offer new opportunities having implications for nanomedicine and nanodiagnostics.