Genetic diversity and differentiation of masu salmon(Oncorhynchus masou masou)between and within cultured populations inferred from microsatellite DNA analysis

作     者：Zhiying JIA Yuyong ZHANG Shuqiang CHEN Lianyu SHI 

作者机构：Heilongjiang River Fisheries Research InstituteChinese Academy of Fishery SciencesHarbin 150070China College of Fisheries and Life ScienceShanghai Ocean UniversityShanghai 201306China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2012年第33卷第S2期

页      面：33-39页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0908[农学-水产] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0833[工学-城乡规划学] 0713[理学-生态学] 0834[工学-风景园林学（可授工学、农学学位）] 

基　　金：HRFRI Basic Science Research Special Funds(2009HSYZX-YY-10) Heilongjiang Natural Science Fund(C201044) Heilongjiang Postdoctoral Sustentation Fund(LRB10-081)。 

主　　题：Oncorhynchus masou masou Microsatellite Genetic diversity Genetic differentiation 

摘      要：Masu salmon,Oncorhynchus masou masou,is one of the most valuable fishery species that has been introduced to China,though to date no studies on the genetic diversity and genetic relationship among hatchery populations has been performed with molecular markers.We undertook such a study and sampled 120 individuals from three hatchery stocks and analyzed 20 microsatellite loci.All loci were polymorphic and a total of 91 alleles were detected.A relatively low level of genetic diversity was revealed with effective number of allele of 3.1094,3.3299 and 3.1894 and expected heterozygosity of 0.6600,0.6648 and 0.6638 in the three stocks,respectively.Deviations from Hardy-Weinberg equilibrium were found due to heterozygote deficit.Accordingly,evidence of genetic bottlenecks were found in the three stocks.An individual assignment test demonstrated that 85%of individuals were correctly assigned into their original stocks.Pairwise Fst revealed that significant differentiation occurred between these three stocks.The results of the study indicated that disequilibrium of genetic structure and differentiation has occurred in all three stocks.This information collectively provides a basis for measures to avoid of loss of genetic diversity and introgression in Chinese aquaculture.