Effect of Zhuang Medicine Feilongzhangxue on the Expression of HMGB1-TLR4 / RANKL-NF-κB Signaling Pathway Related Factors in Osteoclasts

作     者：Qinghuai Zhang Zhiyong Cao Lan Huang Yimin Zhang Zhao Tian Yuzhou Pang 

作者机构：Guangxi Key Laboratory of Applied Fundamental Research of Zhuang MedicineGuangxi University of Chinese MedicineNanning 530200Guangxi ProvinceChina Key Laboratory of Ethnomedicine(Minzu University of China)Ministiy of EducationMinzu University of ChinaBeijing 100081China Department of MedicineHubei University for NationalitiesEnshi 445000Hubei ProvinceChina Department of GuoyitangRuikang Hospital Affiliated to Guangxi University of Traditional Chinese MedicineNanning 530001Guangxi ProvinceChina 

出 版 物：《Journal of Clinical and Nursing Research》 (临床护理研究（英文）)

年 卷 期：2021年第5卷第3期

页      面：83-88页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the open foundation of Key Laboratory of Ethnomedicine(Minzu University of China),Ministry of Education,No.:KLEM-KF2018Z02 Project for Improving Basic Capabilities of Middle-aged and Young Teachers in Guangxi Institutions of Higher Learning(No.:2020KY07016) Guangxi Health Commission Key Laboratory of Applied Fundamental Research of Zhuang Medicine(Guangxi University of Chinese Medicine)[Gui Wei Ke Jiao Fa(2020)No.17] Training Program under“139”Plan for Developing High-level Medical Talents in Guangxi[Gui Wei Ke Jiao Fa(2020)No.15] Traditional Chinese Medicine-Guangxi first-class discipline[No.Gui Jiao Ke Yan(2018)12] 

主　　题：Zhuang medicine Feilongzhangxue Osteoclasts HMGB1 RANK NF-κB 

摘      要：Objective:To study the regulatory effect of feilongzhangxue on the levels of HMGB1-TLR4/RANKL-NF-κB signaling pathway related factors HMGB1,RANKL,rank,TRAF-6 and NF-κBp65 in osteoclasts,so as to explore the mechanism of feilongzhangxue intervention in RA;Methods:The osteoclasts with good activity were randomly divided into blank group,methotrexate control group and Zhuang medicine feilongzhang blood containing serum treatment group,which were divided into OC+blank group,OC+methotrexate control group,OC+Zhuang medicine feilongzhang blood containing serum group;The expression of HMGB1,RANKL,rank,TRAF-6 and NF-κBp65 mRNA was detected by RT-PCR;The protein expressions of HMGB1,RANKL,RANK,TRAF-6 and NF-κBp65 were detected by ***:PCR results showed that:Compared with the blank group,feilongzhangxue could effectively inhibit the expression levels of HMGB1,RANKL,rank,TRAF-6 and NF-κB p65 mRNA in OC cells,and the inhibitory effect was stronger than *** test results showed that:Compared with the blank group and methotrexate group,feilongzhangxue could effectively inhibit the protein expression of HMGB1,RANKL,rank,TRAF-6 and NF-κB p65 in OC ***:The effect of Zhuang medicine feilongzhangxue on hmgbl-tlr4/rankl-nf-κB signaling pathway of osteoclasts is through the regulation of related factors HMGB1,RANKL,rank,TRAF-6 and NF-κB p65,which may be the key mechanism of Zhuang medicine feilongzhangxue on rheumatoid arthritis.