Tumorigenesis and spontaneous metastasis by luciferase-labeled human xenograft osteosarcoma cells in nude mice

作     者：DU Lin XU Wen-ting FAN Qi-ming TU Bing SHEN Yang YAN Wei TANG Ting-ting WANG You 

作者机构：Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 9 Shanghai Key Lab Orthoped Implant Shanghai 200011 Peoples R China Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 9 Dept Orthoped Surg Shanghai 200011 Peoples R China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2012年第125卷第22期

页      面：4022-4030页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 100214[医学-肿瘤学] 10[医学] 

基　　金：the grants from the National Natural Science Foundation of China the Shanghai Science and Technology Development Fund the Program of Key Disciplines of Shanghai Municipal Education Commission the Doctoral Innovation Foundation of Shanghai Jiaotong University 

主　　题：osteosarcoma lung metastasis luciferase in vivo imaging Saos2 cells 

摘      要：Background Osteosarcoma （OS） is the most common primary malignant tumor of bone. Mouse models of human OS can invariably provide greater insight into the complex mechanisms that underlie the development and pathogenesis of this aggressive tumor. Bioluminescence technology favored tracing cancer cells in vivo. In this study, an OS model was described and evaluated using human OS cell line, Saos2, labeled with luciferase （Saos2-1uc）. Methods Saos2 cells were infected by lentivirus loading a firefly luciferase gene. Luciferase expression of Saos2-1uc cells was characterized both in vitro and in vivo. Specific biologic and oncologic features of Saos2-1uc cells were analyzed. The OS was established as orthotopic xenografts in nude mice. Both orthotopic tumors and spontaneous lung metastasis were analyzed. Results Tumorigenesis and spontaneous lung metastasis in nude mice could be monitored in vivo through in vivo imaging system. The enhancement in proliferation, migration and invasion abilities and the attenuation in adhesion ability were observed in Saos2-1uc cells compared with Saos2 cells. Furthermore, there were the up-regulation of Osteocalcin, CCRIO, CXCR1 and ID1 and the down-regulation of ALP, collagen I, CCR1, CCR3, CXCR3, NID and N-cadherin in Saos2-1uc cells compare to Saos2 cells. The rate of spontaneous lung metastasis in Saos2-1uc cells was higher than that in Saos2 cells, although without significant difference. Conclusions Lentivirus transfection may cause alteration of gene expression profiles and further biological functions. This model can be used in the elucidation of molecular mechanisms of tumorigenesis and the screening of new therapeutic agents.