Effect of Metabolites of Fusarium Solani on Tyrosine Phosphorylation in Cultivated Cells of Solarium Tuberosum
Effect of Metabolites of Fusarium Solani on Tyrosine Phosphorylation in Cultivated Cells of Solarium Tuberosum作者机构:M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry Almaty 050012 Kazakhstan Kazan Institute of Biochemistry and Biophysics Kazan Scientific Center Russian Academy of Science Kazan 420111 Russia
出 版 物:《Journal of Life Sciences》 (生命科学(英文版))
年 卷 期:2011年第5卷第10期
页 面:826-830页
学科分类:0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 090501[农学-动物遗传育种与繁殖]
主 题:Potato Fusarium solani 2D-electrophoresis protein phosphorylation on tyrosine glycoprotein.
摘 要:Elicitor-induced phosphorylation of tyrosine residues in proteins of potato was studied. Proteins of crude extract of suspension culture of potato were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed l l and 25 tyrosine-phosphorylated proteins, respectively. Glycoprotein increased the phosphorylation level of most of these proteins.