Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy

作     者：Yang Liu Zhongying Rui Wei Cheng Licheng Song Yunqiang Xu Ruixin Li Xizheng Zhang 

作者机构：Department of OrthopedicsTianjin Medical University General HospitalTianjin 300052China Institute of Medical Service SupportInstitute of Systems EngineeringAcademy of Military SciencesTianjinChina Department of Nuclear MedicineTianjin Medical University General HospitalTianjin 300052China Tianjin Key Laboratory of Oral and Maxillofacial Function ReconstructionTianjin Stomatological HospitalThe Affiliated Stomatological Hospital of Nankai UniversityTianjin 300041China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第2期

页      面：104-117页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 08[工学] 0806[工学-冶金工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：The authors acknowledge financial support from the National Natural Science Foundation of China(11972198 31470935 and 11432016). 

主　　题：femtosecond laser hybrid micro-/nanostructure cell–material interactions osseointegration macrophage polarization 

摘      要：Cell–material interactions during early osseointegration of the bone–implant interface are critical and involve crosstalk between osteoblasts and osteoclasts.The surface properties of titanium implants also play a critical role in cell–material interactions.In this study,femtosecond laser treatment and sandblasting were used to alter the surface morphology,roughness and wettability of a titanium alloy.Osteoblasts and osteoclasts were then cultured on the resulting titanium alloy disks.Four disk groups were tested:a polished titanium alloy(pTi)control;a hydrophilic micro-dislocation titanium alloy(sandblasted Ti(STi));a hydrophobic nano-mastoid Ti alloy(femtosecond laser-treated Ti(FTi));and a hydrophilic hierarchical hybrid micro-/nanostructured Ti alloy[femtosecond laser-treated and sandblasted Ti(FSTi)].The titanium surface treated by the femtosecond laser and sandblasting showed higher biomineralization activity and lower cytotoxicity in simulated body fluid and lactate dehydrogenase assays.Compared to the control surface,the multifunctional titanium surface induced a better cellular response in terms of proliferation,differentiation,mineralization and collagen secretion.Further investigation of macrophage polarization revealed that increased anti-inflammatory factor secretion and decreased proinflammatory factor secretion occurred in the early response of macrophages.Based on the above results,the synergistic effect of the surface properties produced an excellent cellular response at the bone–implant interface,which was mainly reflected by the promotion of early ossteointegration andmacrophage polarization.