The biological responses and mechanisms of endothelial cells to magnesium alloy

作     者：Zhe Hou Maolong Xiang Nuoya Chen Xiao Cai Bo Zhang Rifang Luo Li Yang Xiaoyi Ma Lifeng Zhou Fugui He Hongchi Yu Yunbing Wang 

作者机构：National Engineering Research Center for BiomaterialsSichuan UniversityChengdu 610064China College of Life SciencesSichuan UniversityChengdu 610064China The Fourth People’s Hospital of ChengduChengdu 610036China Beijing Key Laboratory of Cardiac Drug Device Technology and Evidence Based MedicineBeijing 100021China Institute of Biomedical EngineeringWest China School of Basic Medical Sciences&Forensic MedicineSichuan UniversityChengdu 610041China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2021年第8卷第3期

页      面：57-69页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 08[工学] 0806[工学-冶金工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：This research was supported by the National Natural Science Foundation of China(11802190) National Key Research and Development Program(2016YFC1102200) the 111 Project[The Program of Introducing Talents of Discipline to Universities(B16033)]. 

主　　题：magnesium alloy endothelial cells migration adhesion 

摘      要：Due to its good biocompatibility and degradability,magnesium alloy(Mg alloy)has shown great promise in cardiovascular stent applications.Rapid stent re-endothelialization is derived from migrated and adhered endothelial cells(ECs),which is an effective way to reduce late thrombosis and inhibit hyperplasia.However,fundamental questions regarding Mg alloy affecting migration and adhesion of ECs are not fully understood.Here,we evaluated the effects of Mg alloy on the ECs proliferation,adhesion and migration.A global gene expression profiling of ECs co-culturing with Mg alloy was conducted,and the adhesion-and migration-related genes were examined.We found that Mg alloy had no adverse effects on ECs viability but significantly affected ECs migration and adhesion.Co-cultured with Mg alloy extract,ECs showed contractive adhesion morphology and decreased motility,which was supported by the down-regulation of adhesion-related genes(Paxillin and Vinculin)and migration-related genes(RAC 1,Rho A and CDC 42).Accordingly,the re-endothelialization of Mg alloy stent was inhibited in vivo.Our results may provide new inspiration for improving the broad application of Mg alloy stents.