Histamine 3 receptor activation mediates inhibition of acid secretion during Helicobacter-induced gastritis

作     者：Yana Zavros Nisreen Mesiwala Meghna Waghray Andrea Todisco Arthur Shulkes Juanita L Merchant 

作者机构：Departments of Internal Medicine-GastroenterologyUniversity of Michigan Department of Molecular and Cellular PhysiologyUniversity of Cincinnati Department of SurgeryAustin and Repatriation Medical CenterUniversity of Melbourne Departments of Internal Medicine and Molecular and Integrative PhysiologyUniversity of Michigan 

出 版 物：《World Journal of Gastrointestinal Pathophysiology》 (世界胃肠病理生理学杂志（英文版）（电子版）)

年 卷 期：2010年第1卷第5期

页      面：154-165页

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：Supported in part by Public Health Service Grants R37-DK45729(JLM) Michigan Gastrointestinal Peptide Research Center Pilot Feasibility Grant P30-DK34933(YZ) National Health and Medical Research Council of Australia Grant 350234(AS) 

主　　题：Gastrin Somatostatin Histamine Parietal 

摘      要：AIM:To test the hypothesis that histamine 3 receptor (H3R)activation during Helicobacter infection inhibits gastric acid secretion in vivo and in vitro.METHODS:Helicobacter felis(H.felis)infected and uninfected C57Bl/6 mice were infused with either PBS or the H3 receptor antagonist thioperamide(THIO)for 12 wk.After treatment,mice were analyzed for morphological changes and gastric acid content.Total RNA was prepared from the stomachs of each group and analyzed for changes in somatostatin and gastrin mRNA abundance by real time-polymerase chain reaction(RTPCR).Location of H3 receptors in the stomach was analyzed by co-localization using antibodies specific for the H3 receptor and parietal cell marker H + ,K + -ATPase βsubunit. RESULTS:Inflammation and parietal cell atrophy was observed after 12 wk of H.felis infection.Interestingly, treatment with the H3R antagonist thioperamide(THIO) prior to and during infection prevented H.felis-induced inflammation and atrophy.Compared to the uninfected controls,infected mice also had significantly decreased gastric acid.After eradication of H.felis with THIO treatment,gastric acidity was restored.Compared to the control mice,somatostatin mRNA abundance was decreased while gastrin gene expression was elevated during infection.Despite elevated gastric acid levels, after eradication of H.felis with THIO,somatostatin mRNA was elevated whereas gastrin mRNA was suppressed.Immunofluorescence revealed the presence of H3 receptors on the parietal cells,somatostatin-secreting D-cells as well as the inflammatory cells. CONCLUSION:This study shows that during H.felis infection,gastric acidity is suppressed as a consequence of an inhibitory effect on the parietal cell by H3R activation.The stimulation of gastric mucosal H3Rs increases gastrin expression and release by inhibiting release of somatostatin.