Green CoNi_(2)S_(4)/porphyrin decorated carbon-based nanocomposites for genetic materials detection

作     者：Mohammad Reza Saeb Navid Rabiee Farzad Seidi Bahareh Farasati Far Mojtaba Bagherzadeh Eder C.Lima Mohammad Rabiee Mohammad Reza Saeb;Navid Rabiee;Farzad Seidi;Bahareh Farasati Far;Mojtaba Bagherzadeh;Eder C.Lima;Mohammad Rabiee

作者机构：Universitéde LorraineCentraleSupélecLMOPSF57000 MetzFrance Department of ChemistrySharif University of TechnologyTehranIran Provincial Key Lab of Pulp&Paper Sci and Techand Joint International Research Lab of Lignocellulosic Functional MaterialsNanjing Forestry UniversityNanjing 210037China Department of ChemistryIran University of Science and TechnologyTehranIran Institute of ChemistryFederal University of Rio Grande do Sul(UFRGS)Av.Bento Goncalves 9500Postal Box15003ZIP91501-970 Brazil Biomaterial GroupDepartment of Biomedical EngineeringAmirkabir University of TechnologyTehranIran 

出 版 物：《Journal of Bioresources and Bioproducts》 (生物质资源与工程（英文）)

年 卷 期：2021年第6卷第3期

页      面：215-222页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

主　　题：Biosensor Biomedicine Anti-cas9 Green nanocomposite sgRNA 

摘      要：A one-pot synthesis method was conceptualized and implemented to develop green carbon-based nanocomposites working as *** was synthesized to adorn the surface of nanocomposites making them highly sensitive for giving rise to π-π interactions between the genetic materials,proteins and porphyrin *** hydrogen bond formed between the proteins(analytes)and the nitrogen in the porphyrin structure as well as the surface hydroxyl groups was equally *** this context,different forms of porphyrins were incorporated to explore the interrelationship between the surface morphology and the ability of detection of genetic material and/or proteins by the aid of the synthesized *** phenomenon was conceptualized to optimize the interactions between the biomolecules and the substrate by reaching significant biosensor application in the presence of Anti-cas9 protein and sgRNA(concentration changed between 10 and 500 n mol/L).Almost full quenching of fluorescence emission was observed after addition of 300 n mol/L of Anti-cas9 protein and 250 n mol/L of ***,CoNi_(2)S_(4)provided 12%-29%cytotoxicity in both HEK-293 and PC12 cell lines.