Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica

作     者：Xia Wang Jiaxuan Cheng Jiawei Shen Liguo Liu Ningning Li Ning Gao Feng Jiang Qi Jin Xia Wang;Jiaxuan Cheng;Jiawei Shen;Liguo Liu;Ningning Li;Ning Gao;Feng Jiang;Qi Jin

作者机构：NHC Key Laboratory of Systems Biology of PathogensInstitute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China State Key Laboratory of Membrane BiologyPeking-Tsinghua Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第3期

页      面：618-630页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(31870108,32070081,32000080,31725007,31630087,and 31922036) the Beijing Natural Science Foundation(5192019) the CAMS Innovation Fund for Medical Sciences(2016-I2M-1013) the Non-profit Central Institute Fund of Chinese Academy of Medical Sciences(2017PT31049,2018PT51009,and 2018PT31012) the National Key Research and Development Program of China(2019YFA0508904) supported by High-performance Computing Platform of Peking University 

主　　题：Photorhabdus asymbiotica PVC cryo-electron microscopy effector pyrophosphatase 

摘      要：The extracellular contractile injection systems(e CISs)are encoded in the genomes of a large number of bacteria and *** have previously characterized the overall structure of Photorhabdus Virulence Cassette(PVC),a typical member of the e CIS *** resembles the contractile tail of bacteriophages and exerts its action by the contraction of outer sheath and injection of inner tube plus central ***,the biological function of PVC effectors and the mechanism of effector translocation are still *** combining cryo-electron microscopy and functional experiments,here we show that the PVC effectors Pdp1(a new family of widespread d NTP pyrophosphatase effector in e CIS)and Pnf(a deamidase effector)are loaded inside the inner tube lumen in aPeas in the Pod***,we observe that Pdp1 and Pnf can be directly injected into J774 A.1 murine macrophage and kill the target cells by disrupting the d NTP pools and actin cytoskeleton formation,*** results provide direct evidence of how PVC cargoes are loaded and delivered directly into mammalian macrophages.