Development of an efficient plant dual cytosine and adenine editor

作     者：Rongfang Xu Fanna Kong Ruiying Qin Juan Li Xiaoshuang Liu Pengcheng Wei Rongfang Xu;Fanna Kong;Ruiying Qin;Juan Li;Xiaoshuang Liu;Pengcheng Wei

作者机构：Key Laboratory of Rice Genetic Breeding of Anhui ProvinceRice Research InstituteAnhui Academy of Agricultural SciencesHefei 230031China College of AgronomyAnhui Agricultural UniversityHefei 230036China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第9期

页      面：1600-1605页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：funded by the National Natural Science Foundation of China(U19A2022 and 32000284) Natural Science Foundation of Anhui province(2008085QC101 and 2008085MC71) Open Research Fund Program of Anhui Province Key Laboratory of Rice Genetics and Breeding(SDKF-2021-04) Science and Technology Major Projects of Anhui Province(202003a06020009) Key Technology Research Project of Hefei(J2020G44) 

主　　题：base editing CRISPR dual-base editor genome editing rice 

摘      要：An enhanced CDA-like(eCDAL)was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors(CT-CBE).Then,a novel plant dual-base editor version1(pDuBE1)was developed by integrating TadA-8e into *** editing efficiency of pDuBE1 could reach to 87.6%,with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7%in stably transformed plant *** results showed that pDuBE1 could mediate robust dual editing in plant genome,providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.