Proteome landscape and spatial map of mouse primordial germ cells

作     者：Pan Wang Yilong Miao Xiao-Han Li Na Zhang Qilong Wang Wei Yue Shao-Chen Sun Bo Xiong Jie Qiao Mo Li Pan Wang;Yilong Miao;Xiao-Han Li;Na Zhang;Qilong Wang;Wei Yue;Shao-Chen Sun;Bo Xiong;Jie Qiao;Mo Li

作者机构：Center for Reproductive MedicinePeking University Third HospitalBeijing 100191China College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National Clinical Research Center for Obstetrics and GynecologyBeijing 100191China Key Laboratory of Assisted Reproduction(Peking University)Ministry of EducationBeijing 100191China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2021年第64卷第6期

页      面：966-981页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by the National Key Research and Development Program of China(2017YFC1001501,2016YFC1000604,2018YFC1004002) the Foundation for Innovative Research Groups of National Natural Science Foundation of China(81521002)。 

主　　题：PGCs proteome spatial map sex differentiation meiosis 

摘      要：Primordial germ cells(PGCs)are precursors of both male and female gametes as fundamental materials for organism development.The transcriptome,methylome,and chromatin accessibility profiles of PGCs in both mice and humans have been recently reported.However,little is known about the characteristics of PGCs at the protein levels,which directly exert cellular functions.Here,we construct landscapes of both proteome and 3D spatial distribution of mouse PGCs at E11.5,E13.5 and E16.5 days,the three critical developmental windows for PGCs sex differentiation,female meiosis initiation and male mitotic arrest.In each developmental stage of PGCs,nearly 2,000–3,000 proteins are identified,among which specific functional pathways such as oxidative phosphorylation,DNA damage repair,and meiotic cell cycle are involved for different events during PGCs development.Interestingly,by 3D modeling we find that PGCs spatially cluster into around 1,300 nests in genital ridge at E11.5 and the nest number is not increased by the exponential proliferation of PGCs.Comparative analysis of our proteomic data with published transcriptomic data does not show a close correlation,meaning that the practically executive factors are beyond the transcriptome.Thus,our work offers a valuable resource for the systematic investigations of PGC development at protein level and spatial map.