Targeting of anti-microbial proteins to the hyphal surface amplifies protection of crop plants against Phytophthora pathogens
作者机构：Key Laboratory of Plant ImmunityCollege of Plant ProtectionAcademy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095China College of Plant ProtectionChina Agricultural UniversityBeijing 100091China Center for Genome Research and Biocomputing and Department of Botany and Plant PathologyOregon State UniversityCorvallisOR 97331USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2021年第14卷第8期
页 面：1391-1403页
核心收录：
学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学]
基 金：supported by the National Natural Science Foundation,China(31625023,31721004,and 31801715) the National Key R&D Program,China(2018YFD0201000) supported by Oregon State University,United States
主 题：Phytophthora PI3P AMPs transgenic plants disease resistance
摘 要：Phytophthora pathogens are a persistent threat to the world s commercially important agricultural crops,including potato and *** strategies aim at reducing crop losses rely mostly on disease-resistance breeding and chemical pesticides,which can be frequently overcome by the rapid adaptive evolution of *** crops with intrinsic disease resista nee offer a promising alternative and con tinue to be ***,we explored Phytophthora-derived PI3P(phosphatidylinositol 3-phosphate)as a novel control target,using proteins that bind this lipid to direct secreted anti-microbial peptides and proteins(AMPs)to the surface of Phytophthora *** transgenic Nicotiana benthamiana,soybean,and potato plants,significantly enhanced resistance to different pathogen isolates was achieved by expression of two AMPs(GAFP1 or GAFP3 from the Chinese medicinal herb Gastrodia elata)fused with a PI3P-specific binding domain(FYVE).Using the soybean pathogen *** as an example,we demonstrated that the FYVE domain could boost the activities of GAFPs in multiple independent assays,including those performed in vitro,in vivo,and in *** analysis of *** PI3K1 and PI3K2 genes of this pathogen confirmed that the enhanced activities of the targeted GAFPs were correlated with PI3P levels in the ***,our study provides a new strategy that could be used to confer resistance not only to Phytophthora pathogens in many plants but also potentially to many other kinds of plant pathogens with unique targets.
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