Pre-column Derivatization-High Performance Liquid Chromatography for the Detection of Monensin in Livestock and Poultry Meat
作者机构：Shandong China Quality Inspection Co.LtdJining 272000China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2021年第10卷第2期
页 面：93-94页
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
主 题：Pre-column derivatization-high performance liquid chromatography Livestock and poultry meat Monensin
摘 要：[Objectives]A method for the detection of monensin in poultry and livestock meat by pre-column derivatization-high performance liquid chromatography was established.[Methods]The sample was extracted with chloroform,derivatized with trichloroacetic acid and 2,4-dinitrophenylhydrazine,and centrifuged to obtain a purified solution.A C18 chromatographic column(4.6 mm×150 mm,5μm)was used for separation with(1.5%)acetic acid water∶methanol(volume ratio)=1∶9 as the mobile phase using a DAD detector for detection,and the external standard method was adopted for peak area quantification.[Results]Monensin had good linearity in the concentration range of 5.00-200 mg/L,with the linear correlation coefficient r 20.999;the detection limit was 5.00 mg/kg;the relative standard deviation was smaller than 10%;and the recoveries of standard addition experiment were in the range of 75%-110%.[Conclusions]The method has the advantages of simple pretreatment operation,good derivatization effect and fast detection speed,and is suitable for detecting monensin in poultry and livestock meat.
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