Spatiotemporal control of CRISPR/Cas9 gene editing
作者机构：laboratory of Biomaterials and Translational MedicineCenter for NanomedicineThe Third Affiliated HospitalSun Yat-sen UniversityGuangzhouChina lnstitute of Tissue Regeneration Engineering(ITREN)Dankook UniversityCheonanSouth Korea Department of Nuclear MedicineThe Third Affiliated HospitalSun Yat-sen UniversityGuangzhouChina PCFM Lab of Ministry of EducationSchool of Materials Science and EngineeringSun Yat-sen UniversityGuangzhouChina Department of Gynecology and ObstetricsThe Third Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Liver Disease ResearchGuangzhouChina
出 版 物：《Signal Transduction and Targeted Therapy》 (信号转导与靶向治疗（英文）)
年 卷 期：2021年第6卷第7期
页 面：2088-2105页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：This work was supported by the National Key Research and Development Program of China(2019YFA0111300,2016YFE0117100) the Guangdong Provincial Science and Technology Program(International Scientific Cooperation,2018A050506035) the National Natural Science Foundation of China(51903256,21907113,21875289,U1501243) the Science and Technology Program of Guangzhou(202102010225,201704020016) the Guangdong Provincial Pearl River Talents Program(2019QN01Y131) the Thousand Talents Plan,the Guangdong-Hong Kong Joint Innovation Project(2016A050503026) the Major Project on the Integration of Industry,Education and Research of Guangzhou City(201704030123) the Guangdong Innovative and Entrepreneurial Research Team Program(2013S086) partially supported by grants from National Research Foundation,Republic of Korea(2015K1A1A2032163,2018K1A4A3A01064257,2018R1A2B3003446)
主 题：activation breakthrough consequences
摘 要：The clustered regularly interspaced short palindromic repeats(CRISPRJ/associated protein 9(CRISPR/Cas9)gene editing technology,as a revolutionary breakthrough in genetic engineering,offers a promising platform to improve the treatment of various genetic and infectious diseases because of its simple design and powerful ability to edit different loci ***,failure to conduct precise gene editing in specific tissues or cells within a certain time may result in undesirable consequences,such as serious off-target effects,representing a critical challenge for the clinical translation of the ***,some emerging strategies using genetic regulation,chemical and physical strategies to regulate the activity of CRISPR/Cas9 have shown promising results in the improvement of spatiotemporal ***,in this review,we first summarize the latest progress of these advanced strategies involving cell-specific promoters,small-molecule activation and inhibition,bioresponsive delivery carriers,and optical/thermal/ultrasonic/magnetic ***,we highlight the advantages and disadvantages of various strategies and discuss their obstacles and limitations in clinical ***,we propose viewpoints on directions that can be explored to further improve the spatiotemporal operability of CRISPR/Cas9.
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