RT-PCR法检测我国东南沿海凡纳滨对虾的桃拉综合征病毒

作     者：黄新新 莫胜兰 陆承平 HUANG Xin-xin;MO Sheng-lan;LU Cheng-ping

作者机构：南京农业大学农业部动物疫病诊断与免疫重点实验室江苏南京210095 

出 版 物：《中国病毒学》 (Virologica Sinica)

年 卷 期：2005年第20卷第5期

页      面：546-548页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

主　　题：凡纳滨对虾 RT—PCR 桃拉综合征病毒(TSV) 核酸测序 

摘      要：Taura syndrome virus(TSV) is one of the important pathogenic agents of Penaeus vannami,which causes prawn infection with serious disease in China in recent years.245 samples collected from 26 farms of Zhangzhou,Xiamen,Shenzhen,Yangjiang,Ningbo and *** detected for TSV with RT-PCR,the positive rates were 100%,33.3%,40.7%,50% and 40%,*** blotting and nucleotide sequencing were performed to confirm the specificity of the amplified RT-PCR *** homology of 231bp nucleotide sequences reached 98.3%-100% when analysed and compared with the GenBank using the BLAST *** analyses clustered the TSV isolates into two groups: one contained USA and Chinese Taiwan isolates;the other contained Vietnam isolate(YN1) and 21 isolates of China s *** 21 China isolates clustered into one branch,while the YN1 distributed in another branch.5 strains from Guangzhou and 2 strains from Shenzhen constituted one subcluster of the China branch.