Selenogenome and AMPK signal insight into the protective effect of dietary selenium on chronic heat stress-induced hepatic metabolic disorder in growing pigs

作     者：Yan Liu Jiayong Tang Ying He Gang Jia Guangmang Liu Gang Tian Xiaoling Chen Jingyi Cai Bo Kang Hua Zhao Yan Liu;Jiayong Tang;Ying He;Gang Jia;Guangmang Liu;Gang Tian;Xiaoling Chen;Jingyi Cai;Bo Kang;Hua Zhao

作者机构：Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory for Animal Disease-Resistance NutritionMinistry of EducationHuimin RoadWenjiang DistrictChengdu 611130SichuanChina College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130SichuanChina 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第1期

页      面：152-165页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported partly by the National Natural Science Foundation of China(No.31772643) the Special Research Funding for Discipline Construction in Sichuan Agricultural University(No.03570126) 

主　　题：Chronic heat stress Hepatic metabolism HMSeBA Pigs Selenoprotein 

摘      要：Background:Chronic heat stress(CHS)disrupts hepatic metabolic homeostasis and jeopardizes product quality of ***(Se)may regulate the metabolic state through affect ***,we investigate the protective effect of dietary hydroxy-4-methylselenobutanoic acid(HMSeBA)on CHS induced hepatic metabolic disorder in growing pigs,and the corresponding response of ***:Forty crossbreed growing pigs were randomly assigned to five groups:control group raised in the thermoneutral environment(22±2℃)with basal diet;four CHS groups raised in hyperthermal condition(33±2℃)with basal diet and supplied with 0.0,0.2,0.4,and 0.6 mg Se/kg HMSeBA,*** trial lasted 28 *** serum biochemical,hepatic metabolism related enzyme,protein and gene expression and 25 selenoproteins in liver tissue were determined by real-time PCR,ELISA and western ***:CHS significantly increased the rectal temperature,respiration rate,serum aspartate aminotransferase(AST)and low-density lipoprotein cholesterol(LDL-C)of pigs,up-regulated hepatic heat shock protein 70(HSP70)and induced lower liver weight,glycogen content,hepatic glucokinase and glutathione peroxidase(GSH-Px).The CHSinduced liver metabolic disorder was associated with the aberrant expression of 6 metabolism-related gene and 11 selenoprotein encoding genes,and decreased the protein abundance of GCK,GPX4 and *** improved anti-oxidative capacity of liver.0.4 or 0.6 mg Se/kg HMSeBA supplementation recovered the liver weight,glycogen content and rescue of mRNA abundance of genes related to metabolism and protein levels of *** supplementation changed expressions of 15 selenoprotein encoding genes,and enhanced protein expression of GPX1,GPX4 and SELENOS in the liver affected by *** alone showed no impact while HMSeBA supplementation increased protein levels of p-AMPKαin the ***:In summary,HMSeBA supplementation beyond nutrient requirement mitigates CHS-induced hepati