Determination of rare earth elements in plant protoplasts by MAA

作     者：ZHANG Zhiyong WANG Yuqi SUN Jingxin LI Fuliang CHAI Zhifang XU Lei LI Xin CAO Guoyin 

作者机构：Inslilule of High Knergy Physics and Laboratory of Nuclear Analytical TechniquesChinese Academy of SciencesBeijing 100080China Graduate School of the Chinese Academy of Agriculture.Beijing 100081China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2000年第45卷第16期

页      面：1497-1499页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：National Natural Science Foundation of China NSFC: 29871030 

主　　题：protoplast molecular activation analysis rare earth elements. 

摘      要：A preliminary study on the speciation of rare earth elements in plant cells has been carried out by molecular activation analysis (MAA). Mesophyll protoplasts of Brassica napus were isolated by enzymatic digestion. After being washed with isosmotic solution containing EDTA for several times, the protoplasts were purified by gradient centrifugation. Then the concentration of rare earth elements (REEs) in the protoplasts was determined by neutron activation analysis. The result shows that REEs can enter the cells of the plant.