In vitro and in vivo evaluation of implantable bacterial-killing coatings based on host defense peptides and their synthetic mimics

作     者：Yuxin Qian Shuai Deng Xue Wu Yunrui She Runhui Liu Haodong Lin Yuxin Qian;Shuai Deng;Xue Wu;Yunrui She;Runhui Liu;Haodong Lin

作者机构：State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237China Key Laboratory for Ultrafine Materials of Ministry of EducationFrontiers Science Center for Materiobiology and Dynamic ChemistryResearch Center for Biomedical Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai 200237China Department of Orthopedic SurgeryShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2021年第91卷第32期

页      面：90-104页

核心收录：

学科分类：0806[工学-冶金工程] 0817[工学-化学工程与技术] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0703[理学-化学] 0802[工学-机械工程] 0702[理学-物理学] 0801[工学-力学（可授工学、理学学位）] 

基　　金：financially supported by the National Natural Science Foundation of China (Nos.21774031,21861162010and 21574038) the Clinical Research Plan of SHDC (No.SHDC2020CR4024) the Natural Science Foundation of Shanghai (No.18ZR1410300) 

主　　题：Contact-killing Antimicrobial surface HDPs HDP mimic Covalent binding 

摘      要：Contact-killing antimicrobial coatings based on host defense peptides(HDPs) and their synthetic mimics have shown potential as powerful tools to combat implant-associated infections. Covalent modification of the antimicrobial surface has been utilized to prevent early-stage microbial infections owing to the less drug-leaching possibility that is beneficial to human health and the natural environment. Although considerable progress has been achieved in preparing contact-killing antimicrobial surfaces, discussions focusing on the in vitro and in vivo evaluations of these surfaces are limited. In this review, we summarized the established in vitro methods to simulate the practical interaction of microbes with the surrounding biological environment and the reported in vivo studies at different implant sites. We suggested that the in vivo specific site infection model is essential to gain a comprehensive understanding of these antimicrobial coatings in the preclinical stage, which can be established based on investigations performed using various in vitro assays and conventional non-specific site infection models. Overall, these precedent studies focusing on bacterial contact-killing coatings modified with HDPs and HDP mimics can be considered as critical to assess the surface antibacterial ability and to guide the future developments and applications of antimicrobial surfaces.