Relationship between mismatch repair protein, RAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients

作     者：Jun-Zhen Fan Gao-Fei Wang Xue-Bin Cheng Zhou-Huan Dong Xin Chen Yu-Jiao Deng Xin Song 

作者机构：Department of PathologyFirst Medical CenterChinese People's Liberation Army General HospitalBeijing 100853China Department of UltrasoundFirst Medical CenterChinese People's Liberation Army General HospitalBeijing 100853China 

出 版 物：《World Journal of Clinical Cases》 (世界临床病例杂志)

年 卷 期：2021年第9卷第11期

页      面：2458-2468页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Elderly patients Colorectal cancer Mismatch repair protein Gene mutation Expression Diagnosis 

摘      要：BACKGROUND Colorectal cancer(CRC)is common in elderly *** repair(MMR)protein deletion is one of the causes of *** RAS(KRAS/NRAS),BRAF,and PIK3CA genes are important gene targets in CRC treatment and are closely related to the prognosis and survival of ***,little is known regarding the relationship between the expression of MMR,RAS,BRAF,PIK3CA and the clinicopathological features in CRC *** To analyze the relationship between the expression of MMR,RAS,BRAF,PIK3CA and the clinicopathological features in *** A total of 327 elderly patients with CRC were enrolled,and immunohistochemistry was used to detect the MMR ***-time quantitative polymerase chain reaction was used to detect the RAS(KRAS/NRAS),BRAF,and PIK3CA *** clinicopathological data of the patients were recorded and analyzed by SPSS 19.0 statistical *** In 327 elderly patients with CRC,the rate of MMR protein loss was 9.79%(32/327),and the deletion rate of four MMR proteins(MSH2,MSH6,MLH1,PMS2)was 1.83%(6/327),3.06%(10/327),7.65%(25/327),and 7.65%(25/327),*** were no significant differences between MMR protein deletion and sex,pathological type,tumor morphology,differentiation degree or lymph node metastasis(P0.05),but there was a significant difference between MMR protein deletion and tumor diameter and tumor location(P=0.048/P=0.000).The mutation rates of the KRAS,NRAS,BRAF and PIK3CA genes in elderly CRC patients were 44.95%(147/327),2.45%(8/327),3.36%(11/327)and 2.75%(9/327),respectively;the KRAS gene mutation was closely related to tumor morphology(P=0.002)but not to other clinicopathological features(P0.05),and there were no significant differences between NRAS gene mutation and clinicopathological features(P0.05).The BRAF gene mutation showed a significant difference in pathological type,tumor location,differentiation degree and lymph node metastasis(P0.05),but was not correlated with sex,tumor size and tumor