Antitumor effect of recombinant human endostatin combined with cisplatin on rats with transplanted Lewis lung cancer

作     者：Zhan-Wu Yu Ying-Hua Ju Cheng-Liang Yang Han-Bing Yu Quan Luo Ye-Gang Ma Yong-Yu Liu 

作者机构：Department of Thoracic SurgeryLiaoning Cancer Hospital and Institute Dalian Medical University Affiliated Tumour Hospital Department of Biochemistry and Molecular BiologyChina Medical University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2015年第8卷第8期

页      面：652-655页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Liaoning BaiQianWan Talents Program(No.2012921017) 

主　　题：Lewis lung cancer Cisplatin Recombinant human endostatin Vascular endothelial growth factor Microvessel density 

摘      要：Objective: To observe the antitumor effect and mechanism of recombinant human endostatin(Endostar) injection in tumor combined with intraperitoneal injection of cisplatin on subcutaneous transplanted Lewis lung cancer in rats. Methods: A total of 30 C57 rats were selected, and the monoplast suspension of Lewis lung cancer was injected into the left axilla to prepare the subcutaneous transplanted tumor models in the axilla of right upper limb. The models were randomly divided into Groups A, B, and C. Medication was conducted when the tumor grew to 400 mm3. Group A was the control group without any interventional treatment. Group B was injected with Endostar 5 ***-1.d for 10 d. Group C was given the injection of Endostar 5 ***-1.d combined with intraperitoneal injection of cisplatin 5 ***-1.d for 10 d. All the rats in three groups were executed the day after the 10-d medication and the tumor was taken off for measurement of volume and mass changes and calculation of antitumor rate, after which the vascular endothelial growth factor(VEGF) concentration in rats plasma was determined by ELISA. The tumor tissues were cut for the preparation of conventional biopsies. After hematoxylin-eosin staining, the pathologic histology was examined to observe the structures of tumor tissues, VEGF score and microvessel density(MVD) in each group. Results: The volume and mass of tumor in Groups B and C were significantly lower than Group A(P 0.05) while the tumor volume and mass in Group C were significantly lower than Group B(P 0.05). The antitumor rate in Group C was significantly higher than Group B(P 0.05), but the tumor VEGF score, MVD and plasma VEGF level in Group C were significantly lower than Groups A and B(P 0.05). In Group B, the tumor VEGF score, MVD and plasma VEGF level were significantly lower than Group A(P 0.05). The microscopic image of Group C showed that its number of active tumor cells and the blood capillary around tumor was significantly smaller