A functional characterization of TaMs1 orthologs in Poaceae plants

作     者：Jian Li Zheng Wang Zhenyi Chang Hang He Xiaoyan Tang Ligeng Ma Xing Wang Deng Jian Li;Zheng Wang;Zhenyi Chang;Hang He;Xiaoyan Tang;Ligeng Ma;Xing Wang Deng

作者机构：Peking University Institute of Advanced Agricultural SciencesWeifang 261325ShandongChina Shenzhen Institute of Molecular Crop DesignShenzhen 518107GuangdongChina College of Life SciencesCapital Normal UniversityBeijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental ImprovementBeijing 100048China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2021年第9卷第6期

页      面：1291-1300页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by Peking University Institute of Advanced Agricultural Sciences  and Beijing Municipal Government Science Foundation (IDHT20170513) 

主　　题：MS1 Poaceae nsLTP Pollen development 

摘      要：TaMs1 encodes a non-specific lipid transfer protein(nsLTP) and is required for pollen development in wheat. Although MS1 is a Poaceae-specific gene, the roles of MS1 genes in other Poaceae plants are unknown, especially in rice and maize. Here, we identified one ortholog in rice(OsLTPg29) and two orthologs in maize(ZmLTPg11 and ZmLTPx2). Similar to TaMs1, both OsLTPg29 and ZmLTPg11 genes are specifically expressed in the microsporocytes, and both OsLTPg29 and ZmLTPg11 proteins showed lipid-binding ability to phosphatidic acid and several phosphoinositides. To determine their roles in pollen development, we created osltpg29 mutants and zmltpg11 zmltpx2 double mutants by CRISPR/***29, not zmltpg11 zmltpx2, is defective in pollen development, and only OsLTPg29, not ZmLTPg11,can rescue the male sterility of tams1 mutant. Our results demonstrate that the biological function of MS1 in pollen development differs in the evolution of Poaceae plants.