Expression of proline-rich Akt-substrate PRAS40 in cell survival pathway and carcinogenesis

作     者：Gavin PORTER 

作者机构：Department of Pharmacology Emory University School of Medicine Atlanta GA 30032 USA 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2005年第26卷第10期

页      面：1253-1258页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100214[医学-肿瘤学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported in part by Anhui Key Laboratory of Eco-engineering and Biotechnique Fund and National Institutes of Health Grants GM60033-03 

主　　题：PRAS40 PI3K-Akt pathway kinase inhibitors 14-3-3 protein 

摘      要：Aim: To study the expression of proline-rich Akt-substrate PRAS40 in the cell survival pathway and tumor progression. Methods: The effects of three key kinase inhibitors on PRAS40 activity in the cell survival pathway, serum withdrawal,U2O2and overexpression of Akt were tested. The expression of PRAS40, Akt, Raf and 14-3-3 in normal cells and cancer cell lines was determined by Western blot. Results: The PI3K inhibitors worthmannin and Ly294002, but not rapamycin, completely inhibited the phosphorylation of Akt and PRAS40. The phosphorylation level of Akt decreased after serum withdrawal and treatment with the MEK inhibi tor Uo 126, but increased after treatment with H2O2at low concentration, whereas none of these treatments changed PRAS40 activity. 14-3-3 is a PRAS40 binding protein, and the expression of 14-3-3, like that of PRAS40, was higher in HeLa cells than in HEK293 cells; PRAS40 had a stronger phosphorylation activity in A549 and HeLa cancer cells than in HEK293 normal cells. In the breast cancer model (MCF10A/MCF7) and lung cancer model (BEAS/H1198/H1170) we also found the same result: PRAS40 was constitutively active in H1198/H1170 and MCF7 premalignant and malignant cancer cells, but weakly expressed in MCF10A and BEAS normal cell. We also discussed PRAS40 activity in other NSCLC cell lines. Conclusion: The PI3K-Akt survival pathway is the main pathway that PRAS40 is involved in; PRAS40 is a substrate for Akt, but can also be activated by an Akt independent mechanisms. PRAS40 activation is an early event during breast and lung carcinogenesis.