The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)
作者机构：Key Laboratory for Fruit Tree GrowthDevelopment and Quality ControlZhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesZhengzhou 450009China Key Laboratory of Horticultural Plant Biology(Ministry of Education)College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：1301-1315页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：the National Science Foundation of China(Grant No.31801820) the National Key Research and Development Project of China(Grant No.2019YFD1000800) the Special Engineering Science and Technology Innovation,Chinese Academy of Agricultural Sciences(Grant No.CAAS-ASTIP-2015-ZFRI) the Modern Agricultural Industrial Technology System of Henan Province(Grant No.S2014-11)
主 题：expression freezing fir
摘 要：Beta-amylase(BAM)plays an important role in plant resistance to cold ***,the specific role of the BAM gene in freezing tolerance is poorly *** this study,we demonstrated that a cold-responsive gene module was involved in the freezing tolerance of *** this module,the expression of AaBAM3.1,which encodes a functional protein,was induced by cold ***3.1-overexpressing kiwifruit lines showed increased freezing tolerance,and the heterologous overexpression of AaBAM3.1 in Arabidopsis thaliana resulted in a similar *** results of promoter GUS activity and cis-element analyses predicted AaCBF4 to be an upstream transcription factor that could regulate AaBAM3.1 *** investigation of protein-DNA interactions by using yeast one-hybrid,GUS coexpression,and dual luciferase reporter assays confirmed that AaCBF4 directly regulated AaBAM3.1 *** addition,the expression of both AaBAM3.1 and AaCBF4 in kiwifruit responded positively to cold ***,we conclude that the AaCBF-AaBAM module is involved in the positive regulation of the freezing tolerance of kiwifruit.
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