Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis

作     者：Peng Cui Wanfei Liu Yuhui Zhao Qiang Lin Daoyong Zhang Feng Ding Chengqi Xin Zhang Zhang Shuhui Song Fanglin Sun Jun Yu Songnian Hu 

作者机构：CAS Key Laboratory of Genome Sciences and InformationBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100029China Institute of Epigenetics and Cancer ResearchSchool of MedicineTsinghua UniversityBeijing 100080China Computational Bioscience Research Center4700 King Abdullah University of Science and TechnologyThuwa123955-6900Kingdom of Saudi Arabia Graduate University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2012年第10卷第2期

页      面：82-93页

核心收录：

学科分类：0710[理学-生物学] 0601[历史学-考古学] 06[历史学] 1001[医学-基础医学(可授医学、理学学位)] 060109[历史学-专门考古] 0905[农学-畜牧学] 09[农学] 0714[理学-统计学（可授理学、经济学学位）] 0703[理学-化学] 090501[农学-动物遗传育种与繁殖] 0701[理学-数学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by Grants from Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-R-01-04) National Science and Technology Key Project (2008ZX1004-013) 863 Program(2009AA01A130) Special Foundation Work Program(2009FY120100) National Key Technology R&D Program (2008BA164B02) 973 Program (2011CB944100,2011CB965300 and 2007CB948101) from the Ministry of Science and Technology of the People’s Republic of China 

主　　题：H3K4me3 H3K27me3 Mouse 

摘      要：The global features of H3K4 and H3K27 trimethylations （H3K4me3 and H3K27me3） have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genorne-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChlP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLID technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. How- ever, the bivalent domains tend towards a ＂winner-takes-all＂ approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a ＂winner-takes-all＂ principle to regulate gene expression.