Isolation and identifi cation of antimicrobial metabolites from sea anemone-derived fungus Emericella ***01

作     者：Yang YUE Huahua YU Rongfeng LI Linfeng HU Song LIU Rong’e XING Pengcheng LI Yang YUE;Huahua YU;Rongfeng LI;Linfeng HU;Song LIU;Rong'e XING;Pengcheng LI

作者机构：Key Laboratory of Experimental Marine BiologyCenter for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Drugs and BioproductsPilot Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China School of Chemistry and Chemical EngineeringHenan Institute of Science and TechnologyXinxiang 453003China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2021年第39卷第3期

页      面：1010-1019页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：Supported by the National Natural Science Foundation of China(Nos.41376004,41406152) the Key Research and Development Project of Shandong Province(No.2018GHY115008) the Youth Innovation Promotion Association of CAS,and the Scientific and Technological Innovation Project(No.2015ASKJ02) 

主　　题：sea anemone symbiotic fungi phenazine-1-carboxylic acid antimicrobial activity 

摘      要：Marine symbiotic fungi represent an intriguing source of discovery of novel secondary metabolites with various biological *** anemones are benthic marine invertebrates,however,the cultivable symbiotic fungi residing in the sea anemones are paid few attentions compared to those derived from their cnidarian *** we show the identification of antimicrobial secondary metabolites from the sea anemone-derived symbiotic *** offive isolated fungal strains,only the strain SMA01 showed strong antimicrobial activities,which was assigned into the genus Emericella based on the morphological characteristics and the ITS *** swift from liquid fermentation to solid rice medium presented little influence on its antibacterial activity.A chemical investigation of the ethyl acetate extract of the Emericella ***01 led to discovery of the primary antibiotic metabolite phenazine-1-carboxylic *** IC_(50) values of the phenazine-1-carboxylic acid against Phytophthora capsici,Gibberella zeae,and Verticillium dahliae were determined to be 23.26-53.89μg/*** the best of our knowledge,this was the first report of Emericella *** sea *** current study may benefit understanding of the defensive chemical interactions between the symbiotic fungi and their host sea anemones.