Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

作     者：Jiaqi Liu Jie Li Wentao Chen Xintao Xie Xingang Chu Teresa G.Valencak Yizhen Wang Tizhong Shan Jiaqi Liu;Jie Li;Wentao Chen;Xintao Xie;Xingang Chu;Teresa G.Valencak;Yizhen Wang;Tizhong Shan

作者机构：College of Animal SciencesZhejiang UniversityHangzhouChina Key Laboratory of Molecular Animal Nutrition(Zhejiang University)Ministry of EducationHangzhouChina Key Laboratory of Animal Feed and Nutrition of Zhejiang Province866 Yuhangtang RoadHangzhouChina 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2021年第12卷第3期

页      面：891-903页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：The project was partially supported by the National Natural Science Foundation of China(31722053,31672427) the Natural Science Foundation of Zhejiang Province(LR17C170001) the“Hundred Talents Program”funding from Zhejiang University awarded to TZS 

主　　题：CRTC3 Energy metabolism Metabolomics Overexpression Subcutaneous adipocytes Transcriptomics 

摘      要：Background:Meat quality is largely driven by fat deposition,which is regulated by several genes and signaling *** cyclic adenosine monophosphate(cAMP)-regulated transcriptional coactivator 3(CRTC3)is a coactivator of cAMP response element binding protein(CREB)that mediates the function of protein kinase A(PKA)signaling pathway and is involved in various biological processes including lipid and energy ***,the effects of CRTC3 on the metabolome and transcriptome of porcine subcutaneous adipocytes have not been studied ***,we tested whether porcine CRTC3 expression would be related to fat deposition in Heigai pigs(a local fatty breed in China)and Duroc×Landrace×Yorkshire(DLY,a lean breed)pigs in *** effects of adenovirus-induced CRTC3 overexpression on the metabolomic and transcriptomic profiles of subcutaneous adipocytes were also determined in vitro by performing mass spectrometry-based metabolomics combined with RNA sequencing(RNA-seq).Results:Porcine CRTC3 expression is associated with fat deposition in *** addition,CRTC3 overexpression increased lipid accumulation and the expression of mature adipocyte-related genes in cultured porcine subcutaneous *** to the metabolomic analysis,CRTC3 overexpression induced significant changes in adipocyte lipid,amino acid and nucleotide metabolites in *** RNA-seq analysis suggested that CRTC3 overexpression alters the expression of genes and pathways involved in adipogenesis,fatty acid metabolism and glycerophospholipid metabolism in ***:We identified significant alterations in the metabolite composition and the expression of genes and pathways involved in lipid metabolism in CRTC3-overexpressing *** results suggest that CRTC3 might play an important regulatory role in lipid metabolism and thus affects lipid accumulation in porcine subcutaneous adipocytes.