The potential role of DEK over-expression in the radiation response of head and neck cancer
The potential role of DEK over-expression in the radiation response of head and neck cancer作者机构：Department of Radiation OncologyThe Ohio State UniversityOH43210USA The Ohio State University Comprehensive Cancer CenterThe Ohio State UniversityOH43210USA Division of Medical OncologyDepartment of Internal MedicineThe Ohio State UniversityOH43221USA
出 版 物：《Radiation Medicine and Protection》 (放射医学与防护（英文）)
年 卷 期：2021年第2卷第1期
页 面：28-32页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：HPV Oropharynx Radiation DEK
摘 要：Objective:To determine if DEK over-expression is associated with radiation resistance in HPV positive head and neck cancer ***:Control and DEK over-expressing keratinocytes and an HPV positive head and neck cancer cell line respectively were irradiated with 2–10 Gy and the impact on cell survival,γ-H2AX(a marker of DNA damage)and RAD51(homologous recombination)were analyzed via clonogenic assays,and immunofluorescence measurements ***:Upon exposure to increasing doses of radiation,DEK over-expression in keratinocytes at 2,4(P0.05,P0.05),and 10 Gy(P0.01)and an HPV positive head and neck cancer cell line at 2,4,8,and 10 Gy(P0.01)led to improved clonogenic cell *** parallel,irradiation decreased the percent ofγ-H2AX foci at 6,24,and 48 h post-irradiation(P0.05,P0.05,and P0.01 respectively)in NIKS,and at 0,6,24,48 h post-irradiation(P0.05,P0.05,P0.01,P0.01)in C-SCC1 cells but enhanced the percent of RAD51 foci in both DEK over-expressing cell lines relative to their respective control cells at all times points(P0.01).Conclusion:These results suggest DEK over-expression contributes to radio-resistance in HPV positive head and neck cancer cells,potentially by improving repair of DNA double strand breaks through homologous *** molecular mechanisms and relevance to in vivo responses needs further investigation.
维普期刊数据库