铜绿假单胞菌利用HinK感知组胺并控制细菌的致病力

作     者：王娅娅 曹荞 曹芹 甘建华 孙宁 杨财广 Taeok Bae 吴敏 蓝乐夫 Yaya Wang;Qiao Cao;Qin Cao;Jianhua Gan;Ning Sun;Cai-Guang Yang;Taeok Bae;Min Wu;Lefu Lan

作者机构：State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China College of Life ScienceNorthwest UniversityXi'an 710069China University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Genetic EngineeringShanghai Public Health Clinical CenterCollaborative Innovation Center of Genetics and DevelopmentSchool of Life SciencesFudan UniversityShanghai 201438China The State Key Laboratory of Chemical Biology and Drug DiscoveryDepartment of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHong Kong 999077China School of Pharmaceutical Science and TechnologyHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Department of Microbiology and ImmunologyIndiana University School of Medicine-NorthwestGary IN 46408USA Department of Biomedical SciencesUniversity of North DakotaGrand Forks ND 58203-9037USA NMPA Key Laboratory for Testing Technology of Pharmaceutical MicrobiologyShanghai Institute for Food and Drug ControlShanghai 201203China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2021年第66卷第11期

页      面：1101-1118,M0004页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071005[理学-微生物学] 

基　　金：supported by the Ministry of Science and Technology(MOST)of China(2016YFA0501503 and 2019ZX09721001-004-003) the National Natural Science Foundation of China(31670136,31870127,and 81861138047) the Science and Technology Commission of Shanghai Municipality(19JC1416400) the State Key Laboratory of Drug Research(SIMM2003ZZ-03)。 

主　　题：铜绿假单胞菌 调节信号 细菌感染 免疫细胞 感染过程 致病力 转录调节因子 致病能力 

摘      要：在感染过程中,细菌刺激宿主的免疫细胞产生和释放组胺.然而,细菌如何对组胺进行应答及其在细菌感染中的作用尚不清楚.本研究发现铜绿假单胞菌的转录调节因子HinK在细菌对组胺进行应答的过程中起着决定性的作用.HinK直接激活组胺摄取和代谢、铁获取及假单胞菌喹诺酮类信号生物合成等相关基因的表达.在含组胺的培养基中,HinK的转录激活活性受到强烈的诱导;进一步的研究发现组胺代谢物咪唑-4-乙酸与HinK结合从而激活HinK的转录调控活性.基于细菌感染动物模型,发现组胺可增强铜绿假单胞菌的致病能力,同时,HinK基因的缺失导致铜绿假单胞菌的致病能力显著下降.这些结果揭示了细菌利用宿主调节信号分子促进致病力的一种新机制.