The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer(NK)cells do not predict the repeated implantation failure(RIF)for the in vitro fertilization patients

作     者：Hongzhan Zhang Chunyu Huang Xian Chen Longfei Li Su Liu Yuye Li Yongnu Zhang Yong Zeng Lina HuDepartment of Obstetrics and Gynecology The Second Affiliated Hospital of Chongqing Medical 

作者机构：UniversityChongqing400010PR China Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantationShenzhen ZhongshanInstitute for Reproduction and GeneticsFertility CenterShenzhen Zhongshan Urology HospitalShenzhenGuangdong518045PR China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2020年第7卷第2期

页      面：283-289页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 0703[理学-化学] 10[医学] 

基　　金：This work was supported by the Shenzhen Healthcare Research Project,China(SZXJ2018004) Clinical Research Program of Chinese Medical Association,China(17020340703) the Sanming Project of Medicine in Shenzhen,China(SZSM201502035). 

主　　题：Activating receptor Inhibitory receptor NK cytotoxicity Peripheral blood NK cells Repeated implantation failure 

摘      要：Natural killer(NK)cells are thought to play a key role in the successful establishment of a pregnancy by facilitating immunological adaptation of the semi-allogeneic developing embryo.The aim of this study was to explore the cell number,immunophenotypic characteristics,and activities of peripheral blood NK cells in women with repeated implantation failure(RIF).Peripheral blood was obtained from 27 women with RIF and 11 healthy,fertile controls during the middle luteal phase of the menstrual cycle.CD3^-CD56^+NK cells were quantified and analyzed by flow cytometry for the expression of cytolytic molecules(granzyme B,granulysin,and perforin)as well as cell surface receptors responsible for NK cell activation or inhibition(NKG2D,NKp30,NKp46,CD158a,CD158b).NK cytotoxicity was measured at three effector-to-target cell ratios.Women with RIF and fertile controls did not differ significantly in the percentage of circulating CD3CD56t NK cells,or in the proportions of these cells that expressed granzyme B,granulysin,or perforin.The two groups also did not differ significantly in the proportions of NK cells expressing the receptors NKG2D,NKp30,NKp46,CD158a or CD158b.General linear model analysis showed that NK cytotoxicity increased with effector-to-target cell ratio.However,NK cytotoxicity did not differ significantly between patients with RIF and fertile controls.These results suggest that RIF is not associated with significant alterations in the number or function of peripheral blood NK cells.