Discussion on Serum Technique and Cryopreservation Technique in the Culture of Liver Sinusoidal Endothelial Cells
作者机构：Guangxi University of Chinese MedicineNanning 530200China Guangxi Health Technical Vocational CollegeNanning 530023China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2021年第10卷第1期
页 面：129-132,135页
学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：Supported by National Nature Science Foundation of China(8196076181960751,81902764) Guangxi Natural Science Foundation(2020JJA140257) The Training Project for Excellent Middle-aged/Young Teachers in Guangx Higher Education Institutions(2018KY1149)
主 题：Liver sinusoidal endothelial cell Cell culture Serum Cell cryopreservation
摘 要：Hepatic sinusoidal endothelial cells are a kind of highly differentiated cells in hepatic sinusoids, which play an important role in the occurrence and development of liver fibrosis. Hepatic sinusoidal endothelial cells are often used in the study of liver fibrosis. In the process of culturing liver sinusoidal endothelial cells, the treatment of serum for culture and cryopreservation of cells are relatively complicated and error-prone. If the technical details of serum treatment and cell cryopreservation are not handled properly, it will have a more obvious effect on the effect of hepatic sinusoidal endothelial cell culture. We have gained experience in the theoretical study of liver fibrosis and the operation of cell culture experiments, and this paper summarized the details of liver sinusoidal endothelial cell culture such as the problems of serum preservation, thawing, precipitates and heat inactivation, as well as methods and precautions for cell cryopreservation.
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