Magnesium incorporated chitosan based scaffolds for tissue engineering applications

作     者：Udhab Adhikari Nava PRijal Shalil Khanal Devdas Pai Jagannathan Sankar Narayan Bhattarai 

作者机构：Department of Mechanical EngineeringNorth Carolina A&T State UniversityGreensboroNCUSA Department of ChemicalBiological and BioengineeringNorth Carolina A&T State UniversityGreensboroNCUSA Department of Energy and Environmental SystemsNorth Carolina A&T State UniversityGreensboroNCUSA NSF ERC for Revolutionizing Metallic BiomaterialsNorth Carolina A&T State UniversityGreensboroNCUSA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2016年第1卷第2期

页      面：132-139页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 10[医学] 

基　　金：This work is supported financially by the National Science Foundation through Engineering Research Center for Revolutionizing Metallic Biomaterials(ERC-0812348) Nanotechnology Undergraduate Education(NUE-1242139). 

主　　题：Chitosan Magnesium gluconate Carboxymethyl chitosan Tissue engineering Composite scaffolds 

摘      要：Chitosan based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo,controllable degradation rate and tailorable mechanical properties.This paper presents a study of the fabrication and characterization of bioactive scaffolds made of chitosan(CS),carboxymethyl chitosan(CMC)and magnesium gluconate(MgG).Scaffolds were fabricated by subsequent freezing-induced phase separation and lyophilization of polyelectrolyte complexes of CS,CMC and MgG.The scaffolds possess uniform porosity with highly interconnected pores of 50-250 μm size range.Compressive strengths up to 400 kPa,and elastic moduli up to 5 MPa were obtained.The scaffolds were found to remain intact,retaining their original threedimensional frameworks while testing in in-vitro conditions.These scaffolds exhibited no cytotoxicity to 3T3 fibroblast and osteoblast cells.These observations demonstrate the efficacy of this new approach to preparing scaffold materials suitable for tissue engineering applications.