Graphene oxide-composited chitosan scaffold contributes to functional recovery of injured spinal cord in rats

作     者：Bing Yang Pang-Bo Wang Ning Mu Kang Ma Shi Wang Chuan-Yan Yang Zhong-Bing Huang Ying Lai Hua Feng Guang-Fu Yin Tu-Nan Chen Chen-Shi Hu Bing Yang;Pang-Bo Wang;Ning Mu;Kang Ma;Shi Wang;Chuan-Yan Yang;Zhong-Bing Huang;Ying Lai;Hua Feng;Guang-Fu Yin;Tu-Nan Chen;Chen-Shi Hu

作者机构：College of Biomedical EngineeringSichuan UniversityChengduSichuan ProvinceChina Department of NeurosurgerySouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第9期

页      面：1829-1835页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China,No.2018YFC1106800(to ZBH and GFY) Sichuan Science and Technology Project of China,No.2018JY0535(to ZBH) Talents Training Program of Army Medical University of China,No.2019MPRC021/SWH2018QNWQ-05(to TNC) Research on Key Technologies of Photoelectromagnetic Acoustic Intensity Brain of China,No.AWS16J025(to HF) 

主　　题：angiogenesis chitosan electrical conduction graphene oxide regeneration repair scaffold spinal cord injury 

摘      要：The study illustrates that graphene oxide nanosheets can endow materials with continuous electrical conductivity for up to 4 weeks. Conductive nerve scaffolds can bridge a sciatic nerve injury and guide the growth of neurons;however, whether the scaffolds can be used for the repair of spinal cord nerve injuries remains to be explored. In this study, a conductive graphene oxide composited chitosan scaffold was fabricated by genipin crosslinking and lyophilization. The prepared chitosan-graphene oxide scaffold presented a porous structure with an inner diameter of 18–87 μm, and a conductivity that reached 2.83 mS/cm because of good distribution of the graphene oxide nanosheets, which could be degraded by peroxidase. The chitosan-graphene oxide scaffold was transplanted into a T9 total resected rat spinal cord. The results show that the chitosan-graphene oxide scaffold induces nerve cells to grow into the pores between chitosan molecular chains, inducing angiogenesis in regenerated tissue, and promote neuron migration and neural tissue regeneration in the pores of the scaffold, thereby promoting the repair of damaged nerve tissue. The behavioral and electrophysiological results suggest that the chitosan-graphene oxide scaffold could significantly restore the neurological function of rats. Moreover, the functional recovery of rats treated with chitosangraphene oxide scaffold was better than that treated with chitosan scaffold. The results show that graphene oxide could have a positive role in the recovery of neurological function after spinal cord injury by promoting the degradation of the scaffold, adhesion, and migration of nerve cells to the scaffold. This study was approved by the Ethics Committee of Animal Research at the First Affiliated Hospital of Third Military Medical University(Army Medical University)(approval No. AMUWEC20191327) on August 30, 2019.