A longitudinal study of SARS-CoV-2-infected patients reveals a high correlation between neutralizing antibodies and COVID-19 severity

作     者：Vincent Legros Solène Denolly Manon Vogrig Bertrand Boson Eglantine Siret Josselin Rigaill Sylvie Pillet Florence Grattard Sylvie Gonzalo Paul Verhoeven Omran Allatif Philippe Berthelot Carole Pélissier Guillaume Thiery Elisabeth Botelho-Nevers Guillaume Millet Jérôme Morel Stéphane Paul Thierry Walzer François-Loïc Cosset Thomas Bourlet Bruno Pozzetto 

作者机构：CIRI–Centre International de Recherche en InfectiologieTeam EVIRUniv LyonUniversitéClaude Bernard Lyon 1InsermU1111CNRSUMR5308ENS Lyon46 allée d’ItalieF-69007LyonFrance Universitéde LyonVetAgro SupMarcy-l’ÉtoileFrance Department of Infectious Agents and HygieneUniversity-Hospital of Saint-EtienneSaint-EtienneFrance Department of ImmunologyUniversity-Hospital of Saint-EtienneSaint-EtienneFrance CIRI–Centre International de Recherche en InfectiologieTeam GIMAPUniv LyonUniversitéClaude Bernard Lyon 1InsermU1111CNRSUMR5308ENS Lyon46 allée d’ItalieF-69007LyonFrance Department of Infectious DiseasesUniversity-Hospital of Saint-EtienneSaint-EtienneFrance Department of Occupational MedicineUniversity-Hospital of Saint-EtienneSaint-EtienneFrance Department of Intensive Care and Resuscitation(Réanimation G)University-Hospital of Saint-EtienneSaint-EtienneFrance Laboratoire Interuniversitaire de Biologie de la MotricitéUniversitéde LyonUniversitéJean MonnetSaint-EtienneFrance Department of Anesthesiology and Critical CareUniversity-Hospital of Saint-EtienneSaint-EtienneFrance 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2021年第18卷第2期

页      面：318-327页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：The laboratory of FLC received financial support from the LabEx Ecofect(ANR-11-LABX-0048) the“Universitéde Lyon,”within the program“Investissements d’Avenir”(ANR-11-IDEX-0007) operated by the French National Research Agency(ANR),the ANR(grant from RA-Covid-19) the Fondation pour la Recherche Médicale(FRM),and Inserm Transfert.We thank D.Lavillette for providing the SARS-CoV-2 spike expression vector and B.La Scola for providing a clone of Vero-E6 cells.We acknowledge the contribution of SFR Biosciences(UMS3444/CNRS,US8/Inserm,ENS de Lyon,UCBL)ANIRA-Cytometry facility for excellent technical assistance and support 

主　　题：Antibody Pathogenesis Humoral response COVID-19 SARS-CoV-2v 

摘      要：Understanding the immune responses elicited by SARS-CoV-2 infection is critical in terms of protection against reinfection and,thus,for public health policy and vaccine development for *** this study,using either live SARS-CoV-2 particles or retroviruses pseudotyped with the SARS-CoV-2 S viral surface protein(Spike),we studied the neutralizing antibody(nAb)response in serum samples from a cohort of 140 SARS-CoV-2 qPCR-confirmed infections,including patients with mild symptoms and also more severe forms,including those that required intensive *** show that nAb titers correlated strongly with disease severity and with anti-spike IgG ***,patients from intensive care units exhibited high nAb titers;conversely,patients with milder disease symptoms had heterogeneous nAb titers,and asymptomatic or exclusive outpatient-care patients had no or low *** found that nAb activity in SARS-CoV-2-infected patients displayed a relatively rapid decline after recovery compared to individuals infected with other ***,we found an absence of cross-neutralization between endemic coronaviruses and SARS-CoV-2,indicating that previous infection by human coronaviruses may not generate protective nAbs against ***,we found that the D614G mutation in the spike protein,which has recently been identified as the current major variant in Europe,does not allow neutralization ***,our results contribute to our understanding of the immune correlates of SARS-CoV-2-induced disease,and rapid evaluation of the role of the humoral response in the pathogenesis of SARS-CoV-2 is warranted.