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Chemical Research in Chinese Universities 2005年第4期21卷 426-430页

作者： HUANGCheng-zhi FENGPing LIYuan-fang ZHAOHua-wen CollegeofChemistryandChemicalEngineering SouthwestUniversityChongqing400715P.R.China

A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%.

关键词： Total internal-reflected resonance light scattering(TIR-RLS) Protein Evans Blue(EB) Cetyltrimethylammonium bromide(CTMAB)

First Principles Study of AI-Li Intermetallic Compounds

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Chinese Journal of Chemical Physics 2012年第6期25卷 659-665,I0003页

作者： Hai-li Yu Xiao-hui Duan Yong-jun Ma Min Zeng State Key Laboratory Cultivation Base for Nonmetal Composites and Functional Materials :SouthwestUniversity of Science and Technology Mianyang 621010 China

The structural properties, heats of formation, elastic properties, and electronic structures of four compositions of binary A1-Li intermetallics, A13Li, A1Li, A12Li3, and A14Li9, are ana- lyzed in detail by using density functional theory. The calculated formation heats indicate a strong chemical interaction between A1 and Li for all the A1-Li intermetallics. In partic- ular, in the Li-rich A1-Li compounds, the thermodynamic stability of intermetallics linearly decreases with increasing concentration ***. According to the computational single crystal elastic constants, all the four A1-Li intermetallic compounds considered here are mechani- cally stable. The polycrystalline elastic modulus and Poisson＇s ratio have been deduced by using Voigt, Reuss, and Hill approximations, and the calculated ratios of bulk modulus to shear modulus indicate that the four compositions of binary A1-Li intermetallics are brittle materials. With the increase of Li concentration, the bulk modulus of A1-Li intermetallics decreases in a linear manner.

关键词： First principles A1-Li intermetallic compound Mechanical property Forma-tion heat Elastic properties

Research on Financial Support of New Countryside Construction in China

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International English Education Research 2017年第4期 78-80页

作者： ZHOU Danyang southwestuniversity chongqing

The cpc＇s 13th Five-Year comprehensive plan put forward a major historic task of building a new socialist countryside. This is the comprehensive implementation of urban and rural development to increase industrial agriculture, move the city to support the countryside, accelerate urbanization and narrow the gap between urban and rural areas. To solve the ＂three rural issues＂, the comprehensive construction ofa well-offsociety, has a very important strategic role to ease the contradiction of China＇s rural and urban ecnomic structure. In the process of building a new socialist countryside, the financial policy, as an important means ofmacroeconomic regulation and control, should play a leading role in solving the effective allocation ＆funds. Due to various factors affected by the current supply of rural financial market constraints, system defects and environmental constraints, there is a large gap between the level of the existing rural financial services and the requirements of the new rural construction which is to be improved urgently.

关键词： Ecnomic structure financial support New rural construction Urbanization

Molecular Identification and Expression Analysis of GhHYDRA1 Gene, a Homologue of HYDRA1 Gene From Upland Cotton (Gossypium hirsutum L.)

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Agricultural Sciences in China 2011年第1期10卷 41-48页

作者： ZANG Zhen-le HU Ming-yu LI Xian-bi CHEN Kui-jun LIAO Peng XIAO Yue-hua HOU Lei PEI Yanand LUO Ming Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture/Biotechnology Research Center SouthwestUniversity Chongqing 400716 P.R.China

Beside as precursors of BRs biosynthesis, more and more evidences supported that phytosterols play an important role in plant growth and development. To investigate the effects of phytosterols on the fiber development of upland cotton （Gossypium hirsutum L.） and the molecular base of sterol regulating cotton fiber growth, a homologue of HYDRA1 was cloned from upland cotton （cv. Xuzhou 142） by screening cotton fiber EST database and contigging the candidate ESTs. The GhHYDRA1 encoded a polypeptide of 218 amino acid residues and the deduced amino acid sequences had high homology with the members of HYDRA1 in Populus trichocarpa, Solanum tuberosum, and Arabidopsis thaliana. Moreover, GhHYDRA1 had comparable transmembrane regions to AtHYDRA1 in sequence, length, order, and spacing, except for a C-terminal polylysine cluster. Quantitative real-time RT-PCR analysis revealed that the higher expression levels of GhHYDRA1 gene were detected in 6 to 12 DPA （days post anthesis） fibers, while the lower levels were observed in 0 DPA ovule （with fibers） and 16 to 18 DPA fibers. These results indicated that GhHYDRA1 is the homologue of HYDRA1 gene and plays a crucial role in fiber elongation. Furthermore, auxin and BL up-regulated the expression level of GhHYDRA1 while ABA and KT down-regulated the expression level of GhHYDRA1 in cotton ovule and fiber growth. The result suggested that phytosterols play a role in the interaction of plant hormones.

关键词： cotton fiber phytosterols brassinosteroids GhHYDRA1

THE COMBINATION PREDICTION OF TRANSMEMBRANE REGIONS BASED ON DEMPSTER-SHAFER THEORY OF EVIDENCE

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Journal of Electronics(China) 2012年第1期29卷 142-147页

作者： DengXinyang XuPeida DengYong SchoolofComputerandInformationScience SouthwestUniversityChongqing400715China SchoolofElectronicsandInformationTechnology ShanghaiJiaotongUniversityShanghai200240China

Transmembrane proteins are some special and important proteins in cells. Because of their importance and specificity, the prediction of the transmembrane regions has very important theoretical and practical significance. At present, the prediction methods are mainly based on the physicochemical property and statistic analysis of amino acids. However, these methods are suitable for some environments but inapplicable for other environments. In this paper, the multi-sources information fusion theory has been introduced to predict the transmembrane regions. The proposed method is test on a data set of transmembrane proteins. The results show that the proposed method has the ability of predicting the transmembrane regions as a good performance and powerful tool.

关键词： Transmembrane regions Prediction Dempster-Shafer theory of evidence Proteins

Cloning and Expression Analysis of a Brassinosteroid Biosynthetic Enzyme Gene,GhDWF1,from Cotton (Gossypium hirsuturm L.)

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Agricultural Sciences in China 2007年第11期6卷 1297-1305页

作者： LUO Ming XIAO Zhong-yi XIAO Yue-hua LI Xian-bi HOU Lei ZHOU Jian-ping HU Ming-yu PEI Yan Key Laboratory of Biotechnology and Crop Quality Improvement Ministry of Agriculture/Biotechnology Research Center SouthwestUniversity Chongqing 400716 P.R.China

Brassinosteroids （BRs） are an important class of plant steroidal hormones that are essential in a wide variety of physiological processes. To determine the effects of BRs on the development of cotton fibers, through screening cotton fiber EST database and contigging the candidate ESTs, a key gene （GhDWF1） involved in the upstream biosynthetic pathway of BRs was cloned from developing fibers of upland cotton （Gossypium hirsutum L.） cv. Xuzhou 142. The full length of the cloned cDNA is 1 849 bp, including a 37 bp 5＇-untranslated region, an ORF of 1 692 bp, and a 120 bp 3＇-untranslated region. The cDNA encodes a polypeptide of 563 amino acid residues with a predicted molecular mass of 65 kD. The deduced amino acid sequence has high homology with the BR biosynthetic enzyme, DWARF1/DIMINUTO, from rice, maize, pea, tomato, and Arabidopsis. Furthermore, the typical conserved structures, such as the transmembrane domain, the FAD- dependent oxidase domain, and the FAD-binding site, are present in the GhDWF1 protein. The Southern blot indicated that the GhDWF1 gene is a single copy in upland cotton genome. RT-PCR analysis revealed that the highest level of GhDWF1 expression was detected in 0 DPA （day post anthesis） ovule （with fibers） while the lowest level was observed in cotyledon. The GhDWF1 gene presents high expression levels in root, young stem, and fiber, especially, at the fiber developmental stage of secondary cell wall accumulation. Moreover, the expression level was higher in ovules （with fibers） of wildtype （Xuzhou 142） than in ovules of fuzzless-lintless mutant at the same developmental stages （0 and 4 DPA）. The results suggest that the GhDWF1 gene plays a crucial role in fiber development.

关键词： cotton DWARF1 gene fiber brassinosteroids phytosterol

A perliminary sturdy on *** linnaeus and *** meridionali

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西南师范大学学报（自然科学版） 1996年第5期21卷 486-490页

作者：刘勇洪锡钧 DepartmentofLifeScience SouthwestUniversityChongq DepartmentofLifeScience South

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关键词：鲶南方大口鲶 DNA指纹图

Jiuzhaigou Ecotourism Areal System:Temporal Evolution of Entropic Change

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Wuhan University Journal of Natural Sciences 2008年第3期13卷 303-308页

作者： SHEN Xingju SU Chunjiang LI Weiji Institute of Mountain Hazards and Environment ChineseAcademy of Sciences Chengdu 610041 Sichuan China College of Tourism and Historical Culture SouthwestUniversity for Nationalities Chengdu 610041 Sichuan China

The temporal evolution of entropy of Jiuzhaigou ecotourism areal system has been quantitatively analyzed by using equation of system entropy V = k In M - k In C. The computed entropy for Jiuzhaigou ecotourism areal system for 20 years indicates a negative value during 1986-1996, which implies that the system had remained a healthy state during this period. The system entropy value, however, showed a constant increase since 1996 until it turned to be positive in 2000 when the system became chaotic. After 2000 the entropy value declined until 2003 when it escalated once again. By analyzing the causes and process of entropy change of the system, it is argued that fluctuation of entropy value is due to the overloading of tourist service capacity by tourism activities. It is desired to curb the flow of tourist volume and reinforce conservation efforts as well as, so that an ideal balance between conservation and development could be maintained.

关键词： Jiuzhaigou system entropy ecotourism chaotic degree evolution

Investigation on symmetry and characteristic properties of the fragmenting source in heavy-ion reactions through reconstructed primary isotope yields

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Nuclear Science and Techniques 2016年第6期27卷 8-12页

作者： Fang-Fang Duan Xing-Quan Liu Wei-Ping Lin Ryoichi Wada Jian-Song Wang Mei-Rong Huang Pei-Pei Ren Yan-Yun Yang Peng Ma Jun-Bing Ma Shi-Lun Jin Zhen Bai Qi Wang School of Physical Science and Technology SouthwestUniversityChongqing 400715China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China Cyclotron Institute Texas A&M UniversityCollege StationTX 77843USA

In this report,a kinematical focusing technique will be briefly described,and using this technique,the primary hot isotope yields from the multiplicities of evaporated light particles,associated with isotopically identified intermediate mass fragments,are *** energy and characteristic properties of the fragmenting source at the time of the intermediate mass fragment formation are extracted from these reconstructed primary isotope yields using a self-consistent *** extracted density-dependent symmetry energy is further compared with those experimentally extracted from other heavy-ion reactions in literatures.A direct connection between the freeze-out concept and transport model simulations in a multifragmenting regime of heavy-ion collisions is also demonstrated quantitatively in the present work.

关键词：重离子反应同位素对称性特性破碎动态聚焦技术中等质量碎片重构

Genome-wide analysis of Panonychus citrimicro RNAs witha focus on potential insecticidal activity of 4 micro RNAs toeggs and nymphs

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Insect Science 2024年第2期31卷 354-370页

作者： Chuan-Zhen Li Yu-Hang Liu Deng Pan Meng-Hao Xia Qiang Zhang Yu-Chuang Li Guo-Rui Yuan Jin-Jun Wang Wei Dou Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionAcademy of Agricultural SciencesSouthwestUniversityChongqingChina Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education) Academy of Agricultural SciencesSouthwest UniversityChongqingChina

Panonychus citriMcGregor(Acari:Tetranychidae),a destructive citrus pest,causes considerable annual economic losses due to its short lifespan and rapid ***(miRNA)-induced RNA interference is a promising approachfor pest control because of endogenous regulation of pest growth and *** for miRNAs with potential insecticidal activity ***,genome-wide analysis ofmiRNAs at different developmental stages was conducted,resulting in the identificationof 136 miRNAs,including 73 known and 63 novel miRNAs.A total of 17 isomiRNAsand 12 duplicated miRNAs were ***-1 and miR-252-5p were identified asreference miRNAs *** *** on differential expressionanalysis,treatments with miR-let-7a and miR-315 mimics and the miR-let-7a antagomirsignificantly reduced the egg hatch rate and resulted in abnormal egg ***-expression or downregulation of miR-34-5p and miR-305-5p through feeding significantlydecreased the adult eclosion rate and caused molting *** 4 miRNAs,miR-let-7a,miR-315,miR-34-5p,and miR-305-5p,had important regulatory functions and insectici-dal properties in egg hatching and adult *** general,these data advance our un-derstanding of miRNAs in mite biology,which can assist future studies on insect-specificmiRNA-based green pest control technology.

关键词： growth and development microRNAs Panonychus citri referencemiRNAs regulation small RNA libraries