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Pig Reproductive and Respiratory Syndrome Virus (PRRSV) Does Not Attach to Boar Sperm;It Affects Only the Velocity Pattern and the Mobility Pattern

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Journal of Biosciences and Medicines 2024年第4期12卷 216-228页

作者： Néstor Méndez Palacios Netzi Naidí Mendez Palacios Felicitas Vázquez Flores José Alfredo Galicia Domínguez Edgar Guadalupe Beltrán Rosas Maximino Méndez Mendoza Facultad de Medicina Veterinaria and Zootecnia-Benemé rita Universidad Aut&#243noma de Puebla Puebla Mexico Independent Advisor in the Porcine Area Puebla Mexico

The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FMVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 106 sperm, the PRRS virus strain was ATCC-VR-2332 (0, 102, 104 and 106 copies of RNA/mL in triplicate), it was observed daily at the CASA;Hamilton Thorne®. Cells with MT (P 2 and 10X6 (P 2) 162.81 ± 10.65 and (106) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 102, 104 and 106 of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 104 with 83.35 ± 2.41 and 106 with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the ALH there is (P 4 with 8.70 ± .26 and 106 with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ejaculates with the PRRS virus affects sperm motil

关键词： PRRSV Boar Sperms Velocity Pattern and Mobility Pattern

dendritic spine degeneration:a primary mechanism in the aging process

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Neural Regeneration Research 2025年第6期20卷 1696-1698页

作者： Gonzalo Flores Leonardo Aguilar-Hernández Fernado García-Dolores Humberto Nicolini Andrea Judith Vázquez-Hernández Hiram Tendilla-Beltrán Instituto de Fisiología Benemérita Universidad Autónoma de Puebla(BUAP)PueblaMexico Instituto de Ciencias Forenses(INCIFO) Tribunal Superior de Justicia de la Ciudad de México(TSJCDMX).Mexico CityMexico Instituto Nacional de Medicina Genómica(INMEGEN) Mexico CityMexico

Recent reports suggest that aging is not solely a physiological process in living beings;instead, it should be considered a pathological process or disease(Amorim et al., 2022). Consequently, this process involves a wide range of factors, spanning from genetic to environmental factors, and even includes the gut microbiome(GM)(Mayer et al., 2022). All these processes coincide at some point in the inflammatory process, oxidative stress, and apoptosis, at different degrees in various organs and systems that constitute a living organism(Mayer et al., 2022;AguilarHernández et al., 2023).

关键词： aging process stress

Competencies in Computer Security and Resolution of Technological Problems in Higher Education Students

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Journal of Computer and Communications 2024年第4期12卷 171-185页

作者： Esteban Pérez Flores Alejandra Aldrette Malacara Sandra R. Murillo Cano Vittorio Zanella Palacios Facultad de Ciencias Administrativas Universidad Aut&#243noma de Baja California Mexicali M&#233xico decanato de Ingenierí as Universidad Popular Aut&#243noma Del Estado de Puebla Puebla M&#233xico

Starting from the importance of preserving our information and protecting our devices against attacks on their vulnerabilities, this article aims to establish the level of knowledge in computer security and problem-solving of students at a university in northwest Mexico. This research has a quantitative approach where the European Framework of Digital Competencies for Citizenship was used as a reference to identify and establish the level of the competencies that were evaluated. The IKANOS Test was used as a data collection tool. The results show that students know the importance of keeping their devices safe and how valuable the information found on them is. On the other hand, the results also show a considerable percentage of students who do not have the knowledge and are at a basic level of knowledge to solve technical problems with their devices.

关键词： Digital Literacy Cybersecurity DigComp Digital Skills Data Protection

Effect of a Commercial Extract of Green Tea and a Pure Catechin on Two Veillonella strains

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Advances in Microbiology 2024年第6期14卷 340-350页

作者： Jorge A. Yáñez-Santos Vianey Lino-Aguilar Elsa I. Castañeda-Roldan Jorge Giron Lilia Cedillo Centro de deteccin Biomolecular Benemrita Universidad Autnoma de Puebla Puebla Mexico Facultad de Estomatologa Puebla Mexico

The catechin Epigallocatechin-3-O-Gallate (EGCG) which is found in of Green Tea extracts (GTE), displays a variety of microbicidal properties. It is largely believed that EGCG inhibits the growth of cariogenic and periodontopathic bacteria. Objective: In this paper we compared the inhibitory activity of EGCG and a commercial GTE on the growth of Veillonella parvula. Chlorhexidine was used as positive control. Methodology: V. parvula ATCC 10790 and a clinical isolate obtained from a periodontal disease patient were cultured in the presence of EGCG or a commercial GTE, and the measurements of bacterial growth inhibition were compared to the values obtained with 0.12 and 0.2% chlorhexidine. Results: Chlorhexidine inhibited bacterial growth, however in contrast to a previous report, neither EGCG nor the GTE showed any effect on bacterial growth. Conclusions: The data show and confirm that chlorhexidine is a growth inhibitor of V. parvula while EGCG and GTE do not display such effect.

关键词： Veillonella Parvulla Chlorhexidine Green Tea Catechin

Post-fire recovery of Puya raimondii, vegetation and birds in the puna of Huascarán National Park, Perú

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Journal of Mountain Science 2024年第1期21卷 20-32页

作者： Mery L.SUNI Giovana P.VADILLO César ARANA Enoc JARA-PENA Letty SALINAS M.Estela PONCE Paul M.RAMSAY Plant Physiology Laboratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru Natural History Museum Universidad Nacional Mayor de San MarcosLima 15072Peru Applied Phytology Laboratory Faculty of Biological SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru department of Statistics Faculty of Mathematical SciencesUniversidad Nacional Mayor de San MarcosLima 15081Peru department of Chemical and Biological Sciences Universidad de las Américas PueblaSan Andrés Cholula CP Cholula CP 72810Mexico

A large fire of 233 ha in Huascarán National Park in Peru provided an opportunity to compare plant and bird responses in burned and nearby unburned zones of the puna. Heights and live diameters of flagship Puya raimondii rosettes(assigned to four broad developmental phases), plant communities(66 species in 24 families and nine growth forms) and bird communities(77 species in six trophic guilds) were monitored after the fire. Although no mortality was observed, Puya raimondii plants were affected by the fire, losing approximately 60% of their photosynthetic area across all developmental phases, but recovered quickly during the first two years after fire. The comparison of Puya rosette recovery after fire was complicated by the changes in live rosette diameter for unburned plants, which showed plasticity of photosynthetic area linked to seasonal and annual fluctuations in precipitation in this relatively dry environment(decreased by 26% for mature adult plants over the study period). Fire caused an immediate change in the density, biomass and composition of vegetation. Although the species present remained similar, their abundances changed significantly immediately after the fire, with notable reductions in dominant tussock grasses. This provided opportunities for other plants, resulting in higher postfire diversity of plant species, genera, families and growth forms. In turn, the changes in vegetation after fire affected the composition of birds according to their trophic guild. Granivores largely disappeared,generalists were mostly unaffected, and other guilds showed a more complex response. As the vegetation recovered, most displaced birds returned within approximately one year.

关键词： Burning Plant biomass Trophic guilds Andes Giant rosette plants

Random noise stimulation in the treatment of patients with neurological disorders

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Neural Regeneration Research 2022年第12期17卷 2557-2562页

作者： Mateo A.Herrera-Murillo Mario Treviño Elias Manjarrez Facultad de Medicina Plan de Estudios Combinados en Medicina(PECEM)UNAMCircuito InteriorCiudad UniversitariaAv.UniversidadCiudad de MéxicoMéxico Instituto de Neurociencias Universidad de GuadalajaraGuadalajaraJaliscoMéxico Instituto de Fisiología Benemérita Universidad Autónoma de PueblaPueblaMéxico

Random noise stimulation technique involves applying any form of energy(for instance,light,mechanical,electrical,sound)with unpredictable intensities through time to the brain or sensory receptors to enhance sensory,motor,or cognitive functions.Random noise stimulation initially employed mechanical noise in auditory and cutaneous stimuli,but electrical energies applied to the brain or the skin are becoming more frequent,with a series of clinical applications.Indeed,recent evidence shows that transcranial random noise stimulation can increase corticospinal excitability,improve cognitive/motor performance,and produce beneficial aftereffects at the behavioral and psychological levels.Here,we present a narrative review about the potential uses of random noise stimulation to treat neurological disorders,including attention deficit hyperactivity disorder,schizophrenia,amblyopia,myopia,tinnitus,multiple sclerosis,post-stroke,vestibular-postural disorders,and sensitivity loss.Many of the reviewed studies reveal that the optimal way to deliver random noise stimulation-based therapies is with the concomitant use of neurological and neuropsychological assessments to validate the beneficial aftereffects.In addition,we highlight the requirement of more randomized controlled trials and more physiological studies of random noise stimulation to discover another optimal way to perform the random noise stimulation interventions.

关键词： auditory noise mechanical noise neurological disorders neuronal noise noise galvanic vestibular stimulation non-invasive brain stimulation transcranial electrical stimulation transcranial random noise stimulation

Curcuma longa L. extract improves the cortical neural connectivity during the aging process

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Neural Regeneration Research 2017年第6期12卷 875-880页

作者： Gonzalo Flores Laboratorio de Neuropsiquiatría Instituto de Fisiología Benemérita Universidad Autónoma de Puebla

Turmeric or Curcuma is a natural product that has anti-inflammatory, antioxidant and anti-apoptotic pharmacological properties. It can be used in the control of the aging process that involves oxidative stress, inflammation, and apoptosis. Aging is a physiological process that affects higher cortical and cognitive functions with a reduction in learning and memory, limited judgment and deficits in emotional control and social behavior. Moreover, aging is a major risk factor for the appearance of several disorders such as cerebrovascular disease, diabetes mellitus, and hypertension. At the brain level, the aging process alters the synaptic intercommunication by a reduction in the dendritic arbor as well as the number of the dendritic spine in the pyramidal neurons of the prefrontal cortex, hippocampus and basolateral amygdala, consequently reducing the size of these regions. The present review discusses the synaptic changes caused by the aging process and the neuroprotective role the Curcuma has through its anti-inflammatory, antioxidant and anti-apoptotic actions.

关键词： Curcuma longa cortical curcumin hippocampus synaptic antioxidant spine connectivity cortex

Soil Quality Evaluation of Hydrocarbon Polluted Parcels

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Journal of Geoscience and Environment Protection 2022年第9期10卷 27-41页

作者： Margarita Teutli León Marco Antonio Cruz Gómez José Castillo Román Andrés Sánchez Engineering department Benemérita Universidad Autónoma de Puebla (BUAP) Puebla México

For hydrocarbon polluted soils that underwent bioremediation it is important to assess its condition after a period of time, but it is more useful when there is an opportunity of comparison against an unpolluted soil and an untreated polluted one. This paper provides a comparison of three adjacent parcels, being the first clean, the second polluted and bioremediated, the third polluted and left to natural attenuation. Study was conducted determining pH, electrical conductivity, carbonates, soil organic matter, chemical oxygen demand, eight anions, and twelve metals. Data were compared against those references for agricultural soils found in the Mexican NOM-021. A Pearson correlation was applied to find coincidences between the three parcels, obtained results allowed to say that bioremediated parcel allowed for most uniform pH, negligible salinity risk, and medium content of soil organic matter, but treatment has enabled heavy metal accumulation since its values are higher in respect to the other parcels. Natural attenuated parcel has some spots with lower pH, a moderately saline risk, a high content of soil organic matter, and lower content of heavy metals. The clean or unaffected parcel exhibit the higher pH values, a slightly saline condition, soil organic matter ranges from high to very high content, heavy metals content is medium, but no reaching dangerous levels. An important assessment is that bioremediation has enhanced the bioavailability of soil organic matter but it is not similar to the values in the unpolluted parcel.

关键词： Soil Quality Hydrocarbon Pollution Soil Unpolluted Bioremediation Natural Attenuation

Resveratrol effects on neural connectivity during aging

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Neural Regeneration Research 2016年第7期11卷 1067-1068页

作者： Gonzalo Flores Rubén Antonio Vázquez-Roque Alfonso Diaz Instituto de fisiología Benemerita Universidad autónoma de Puebla.14 Sur 6301PueblaMexico departamento de Farmacia Facultad de Ciencias químicasbenemérita Universidad autónoma de PueblaPueblaMexico

Aging has been considered a natural process of any living being.The rate of aging depends on many factors,including genetic and environmental factors.For this reason,many researchers in this field suggest that aging i... 详细信息

关键词： resveratrol connectivity older hippocampus neuronal metabolism cortical Aging cortex PPAR