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Molecular Evolution of the Substrate Specificity of Chloroplastic Aldolases/Rubisco Lysine Methyltransferases in Plants

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Molecular Plant 2016年第4期9卷 569-581页

作者： Sheng Ma Jacqueline Martin-Laffon Morgane Mininno Oceane Gigarel Sabine Brugiere Olivier Bastien Marianne Tardift Stephane Ravanel Claude Alban universite grenoble alpes Laboratoire de Physiologie Cellulaire & Vegetale 38041 Grenoble France CNRS UMR5168 38054 Grenoble France CEA iRTSV Laboratoire de Physiologie Cellulaire & Vegetale 38054 Grenoble France INRA USC1359 38054 Grenoble France universite grenoble alpes 38041 Grenoble France CEA iRTSV Biologie & Grande Echelle 38054 Grenoble France INSERM U1038 38054 Grenoble France

Rubisco and fructose-1,6-bisphosphate aldolases （FBAs） are involved in CO2 fixation in chloroplasts. Both enzymes are trimethylated at a specific lysine residue by the chloroplastic protein methyltransferase LSMT. Genes coding LSMT are present in all plant genomes but the methylation status of the substrates varies in a species-specific manner. For example, chloroplastic FBAs are naturally trimethylated in both Pisum sati- vum and Arabidopsis thaliana, whereas the Rubisco large subunit is trimethylated only in the former spe- cies. The in vivo methylation status of aldolases and Rubisco matches the catalytic properties of AtLSMT and PsLSMT, which are able to trimethylate FBAs or FBAs and Rubisco, respectively. Here, we created chimera and site-directed mutants of monofunctional AtLSMT and bifunctional PsLSMT to identify the molecular determinants responsible for substrate specificity. Our results indicate that the His-Ala/Pro- Trp triad located in the central part of LSMT enzymes is the key motif to confer the capacity to trimethylate Rubisco. Two of the critical residues are located on a surface loop outside the methyltransferase catalytic site. We observed a strict correlation between the presence of the triad motif and the in vivo methylation status of Rubisco. The distribution of the motif into a phylogenetic tree further suggests that the ancestral function of LSMT was FBA trimethylation. In a recent event during higher plant evolution, this function evolved in ancestors of Fabaceae, Cucurbitaceae, and Rosaceae to include Rubisco as an additional substrate to the archetypal enzyme. Our study provides insight into mechanisms by which SET-domain protein methyltransferases evolve new substrate specificity.

关键词： SET-domain protein methyltransferase site-directed mutagenesis chimeric enzyme methylation,molecular evolution green lineage

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Identification of Early Nuclear Target Genes of Plastidial Redox Signals that Trigger the Long- Term Response of Arabidopsis to Light Quality Sh∽ts

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Molecular Plant 2015年第8期8卷 1237-1252页

作者： Lars Dietzel Christine Glaeer Monique Liebers Stefan Hiekel Florence Courtois Olaf Czarneckit Hagen Schlicke Yan Zubo Thomas Borner Klaus Mayer Bernhard Grimm Thomas Pfannschmidt Department of Plant Physiology Institute of General Botany and Plant Physiology Friedrich-Schiller-University of Jena Domburger Strasse 159 07743 JenaGermany MIPS/IBI Helmholtz Zentrum MGnchen German Research Center for Environmental Health (GmbH) Ingolst~dter Landstrasse 1 85764 Neuherberg Germany Institute of Biology/Plant Physiology Humboldt-University Berlin Philippstrasse 13 Building 12 10115 Berlin Germany Institute of Biology Genetics Humboldt-University Berlin Chausseestrasse 117 10115 Berlin Germany present address: Institut fer Molekulare Biowissenschaften Pflanzliche Zellphysiologie Max-von-Laue-Strasse 9 Biozentrum Goethe-Universit~t Frankfurt 60438Frankfurt am Main Germany present address: Zentrum fur Molekulare Biologie an der Universitat zu Heidelberg (ZMBH) Im Neuenheimer Feld 282 69120 Heidelberg Germany present address: universite grenoble-alpes F-38000 Grenoble France present address: CNRS UMR5168 F-38054 Grenoble France present address: CEA iRTSV Laboratoire de Physiologie Cellulaire & Vgetale F-38054 Grenoble France present address: INRA USC1359 F-38054 Grenoble France present address: Plant Reproductive Biology Group Department of Physiology and Ceil-Biology Leibniz Institute of Rant Genetics and Crop Plant Research (IPK)OT Gatersleben Corrensstrasse 3 D-06466 Stadt Seeland Germany present address: KWS Saat AG Grimsehlstrasse 31 37555 Einbeck Germany present address: Dartmouth College Department of Biological Sciences Ufe Sciences Center 78 College Street Hanover NH 03755 USA

Natural illumination conditions are highly variable and because of their sessile life style, plants are forced to acclimate to them at the cellular and molecular level. Changes in light intensity or quality induce changes in the reduction/oxidation （redox） state of the photosynthetic electron chain that acts as a trigger for compen- satory acclimation responses comprising functional and structural adjustments of photosynthesis and metabolism. Such responses include redox-controlled changes in plant gene expression in the nucleus and organelles. Here we describe a strategy for the identification of early redox-regulated genes （ERGs） in the nucleus of the model organism Arabidopsis thaliana that respond significantly 30 or 60 min after the generation of a reduction signal in the photosynthetic electron transport chain. By comparing the response of wild-type plants with that of the acclimation mutant stn7, we could specifically identify ERGs. The results reveal a significant impact of chloroplast redox signals on distinct nuclear gene groups including genes for the mitochondrial electron transport chain, tetrapyrrole biosynthesis, carbohydrate metabolism, and signaling lipid synthesis. These expression profiles are clearly different from those observed in response to the reduction of photosynthetic electron transport by high light treatments. Thus, the ERGs identified are unique to redox imbalances in photosynthetic electron transport and were then used for analyzing potential redox-responsive cis-elements, trans-factors, and chromosomal regulatory hot spots. The data identify a novel redox-responsive element and indicate extensive redox control at transcriptional and chromosomal levels that point to an unprecedented impact of redox signals on epigenetic processes.

关键词： plastidial redox signaling,early redox-regulated gene Arabidopsis thaliana Molecular Plant

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Ultralight and superelastic MXene/reduced graphene oxide aerogels for electromagnetic interference shielding

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Nano Research 2025年第1期18卷 1-9页

作者： Xinfeng Zhou Yang Dai Guoyao Yue Yiman Zhang Lulu Li Zhong-Zhen Yu Peng Min Hao-Bin Zhang 21 State Key Laboratory of Organic-Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China Beijing Key Laboratory of Advanced Functional Polymer Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China present address:BOE Technology Innovation Center BOE Technology Group Co.Ltd.Beijing 100176China Xi’an Modern Chemistry Research Institute Xi’an 710065China

Lightweight aerogels feature multifunctionality and a high porosity,yet accompanied with poor structure recovery under large strain *** this work,we develop an air bubble-ice crystal dual template and annealing strategy to integrate low density and high resilience for the conductive transition metal carbides/nitrides(MXene)composite *** air bubbles and ice crystals synergistically exclude the nanosheets to the gas-liquid interfaces,thereby constructing unique Y-shaped junctions and robust *** annealing process greatly enhances the interlayer *** external load,the Yshaped structures prevent the stress concentration at the junctions by transferring the forces to the skeleton for maintaining structural *** addition,the wrinkled and thick cell walls,together with the enhanced interlayer interactions,endow the aerogel with exceptional structural stability and *** a result,the MXene/reduced graphene oxide(RGO)composite aerogels exhibit superelasticity with reversible compressive strains of up to 95%.In addition,the electron bridging effect of the RGO sheets affords the aerogel to deliver excellent electromagnetic interference shielding performance,as high as 46.3 dB at 2.5 ***,the remarkable reshapeability of the aerogels allows for precise regulation of structure and performance(33.5-75.1 dB)by a simple wetting compression *** summary,this work offers helpful inspirations for developing lightweight and superelasticity aerogels for extensive applications.

关键词： lightweight superelasticity MXene aerogel electromagnetic interference shielding reshapeability

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Transcriptional, post-transcriptional and post-translational regulations of gene expression during leaf polarity formation

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Cell Research 2007年第6期17卷 512-519页

作者： Lin Xu Li YangHai Huang National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China present address: IBMP CNRS Universite Louis Pasteur 67084 Strasbourg France present address: Department of Biology University of Pennsylvania Philadelphia PA 19104 USA

Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shootapical meristem (SAM). Several families of transcription factors are known to play critical roles in promoting adaxial orabaxial leaf fate. Recently, post-transcriptional gene silencing pathways have been shown to regulate the establishmentof leaf polarity, providing novel and exciting insights into leaf development. For example, microRNAs (miR165/166)and a trans-acting siRNA (TAS3-derived tasiR-ARF) have been shown to repress the expression of several key transcrip-tion factor genes. In addition, yet another level of regulation, post-translational regulation, has been revealed recently bystudies on the role of the 26S proteasome in leaf polarity. Although our understanding regarding the molecular mecha-nisms underlying establishment of adaxial-abaxial polarity has greatly improved, there is still much that remains *** review aims to discuss recent progress, as well as the remaining questions, regarding the molecular mechanismsunderlying leaf polarity formation.

关键词： Arabidopsis leaf development polarity formation

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OsWRKY62 is a Negative Regulator of Basal and Xa21-Mediated Defense against Xanthomonas orvzae pv. orvzae in Rice

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Molecular Plant 2008年第3期1卷 446-458页

作者： Ying Peng Laura E. Bartley Xuewei Chen Christopher Dardick Mawsheng Chern Randy Ruan Patrick E. Canlas Pamela C. Ronald Department of Plant Pathology University of California Davis CA 95616 USA present address: Monsanto Corporation Davis CA 95616 USA present address: USDA-ARS Appalachian Fruit Research Station Kearneysville WV 25430 USA

The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae （Xoo）, encodes a receptor-like kinase, Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62. The OsWRKY62 gene encodes two splice variants （OsWRKY62.1 and OsWRKY62.2）. OsWRKY62.1：smGFP2 and OsWRKY62.2：smGFP2 fusion pro- teins partially localize to the nucleus. Transgenic plants overexpressing OsWRKY62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of OsWRKY62.1 suppresses the activation of defenserelated genes. These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.

关键词： rice OsWRKY62 Xa21 defense response.

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Expression, Imprinting, and Evolution of Rice Homologs of the Polycomb Group Genes

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Molecular Plant 2009年第4期2卷 711-723页

作者： Ming Luo Damien Platten Abed Chaudhury W.J. Peacock Elizabeth S. Dennis CSIRO Plant Industry GPO BOX 1600 ACT 2601 Australia present address: Vitagrain Uttara Model Town Dhaka Bangladesh present address: IRRI Los Banos Laguna 4031 Philippines

Polycomb group proteins （PcG） play important roles in epigenetic regulation of gene expression. Some core PeG proteins, such as Enhancer of Zeste （E（z））, Suppressor of Zeste （12）（Su（z）12）, and Extra Sex Combs （ESC）, are conserved in plants. The rice genome contains two E（z）-Iike genes, OsiEZ1 and OsCLF, two homologs of Su（z）12, OsEMF2a and OsEMF2b, and two ESC-like genes, OsFIE1 and OsFIE2. OsFIE1 is expressed only in endosperm; the maternal copy is expressed while the paternal copy is not active. Other rice PcG genes are expressed in a wide range of tissues and are not imprinted in the endosperm. The two E（z）-Iike genes appear to have duplicated before the separation of the dicots and monocots; the two homologs of Su（z）12 possibly duplicated during the evolution of the Gramineae and the two ESC- like genes are likely to have duplicated in the ancestor of the grasses. No homologs of the Arabidopsis seed-expressed PcG genes MEA and FIS2 were identified in the rice genome. We have isolated T-DNA insertion lines in the rice homologs of three PcG genes. There is no autonomous endosperm development in these T-DNA insertion lines. One line with a T-DNA insertion in OsEMF2b displays pleiotropic phenotypes including altered flowering time and abnormal flower organs, suggesting important roles in rice development for this gene.

关键词： Polycomb group protein pseudograin imprinting development evolution panicle.

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Occupancy of avian foraging guilds in soybean fields and borders in Entre Ríos, Argentina: responses to vegetation structure and prey resources

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Avian Research 2020年第4期11卷 398-411页

作者： Andrea P.Goijman Michael J.Conroy Vanina D.Varni Jeffrey J.Thompson María Elena Zaccagnini Instituto de Recursos Biológicos CIRNInstituto Nacional de Tecnología Agropecuaria(CNIA-INTA)HurlinghamBuenos AiresArgentina D.B.Warnell School of Forestry and Natural Resources University of GeorgiaAthensGA 30602USA present address:Athens GAUSA present address:Laboratorio de Genómica Ecológica y Evolutiva IrapuatoGtoMéxico present address:Guyra Paraguay-CONACYT Av.Cnel.Carlos BóvedaParque Ecológico Capital-Viñas CuéAsunciónParaguay present address:Instituto Saite AsunciónParaguay present address:The Ronin Institute MontclairNJUSA present address:Instituto Nacional de Tecnología Agropecuaria(INTA) CABAArgentina

Background:Reconciling agriculture and biodiversity conservation is a challenge given the growing demand for agricultural *** recent decades,Argentina has witnessed agricultural expansion and intensification affecting biodiversity and associated ecosystem *** agroecosystems,the level of habitat quality is critical for birds,and may depend on vegetation structure,availability of invertebrate prey,and the use of *** the relationship between vegetation structure and avian occurrence has been widely studied,to our knowledge,there are no studies that also incorporate prey availability throughout the cycle of soybean crops in *** estimated and predicted the effects of land cover and temporal variation on the occurrence of avian foraging guilds in Entre Ríos,Argentina,in order to guide management related to potential ecosystem services provided by *** also estimated temporal effects of vegetation structure and insecticides on the main arthropod orders consumed by birds to evaluate prey ***:We conducted bird and arthropod surveys for 2 years along transects located in 20 randomly selected soybean fields(N=60)and their adjacent borders(N=78)throughout the crop growing season,in four *** estimated avian occupancy,accounting for imperfect detection,and arthropod counts fitting generalized linear mixed ***:The number of native trees in field borders positively influenced the occurrence of most bird species,mainly *** foliage gleaners,also were positively affected by grass *** and aerial foragers were weakly affected by distance to forest and native *** general,the availability of invertebrates to birds was highest during the third *** counts in borders were greater during the last three crop stages than during the pre-sowing ***:We found that with 10 to 15 native tree species in borders,coupled with a complex vegetation structure w

关键词： Agriculture Birds Ecosystem services Insecticide Insectivores Invertebrates Management Native trees Occurrence

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A Double-Phase High-Frequency Traveling Magnetic Field Developed for Contactless Stirring of Low-Conducting Liquid Materials

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Computer Modeling in Engineering & Sciences 2021年第5期127卷 469-486页

作者： Xiaodong Wang Ernst Roland Fautrelle Yves College of Material Science and Opto-Electronic Technology University of the Chinese Academy of ScienceBeijingChina Guangwei(Zhongshan)Intelligent Technology Co. Ltd.Huairou Science CityBeijingChina SIMAP/EPM Universite Grenoble AlpesGrenobleFrance

The use of low electrically conducting liquids is more and more *** is the case for molten glass,salt or slag processing,ionic liquids used in biotechnology,batteries in energy storage and *** present paper deals with the design of a new electromagnetic induction device that can heat and stir low electricallyconducting *** consists of a resistance-capacity-inductance circuit coupled with a low-conducting liquid *** device is supplied by a unique electric power source delivering a single-phase high frequency electric *** main working principle of the circuit is based on a double oscillating circuit inductor connected to the solid-state transistor *** technique,which yields a set of coupled oscillating circuits,consists of coupling a forced phase and an induced phase,neglecting the influence of the electric parameters of the loading part(i.e.,the low-conductivity liquid).It is shown that such an inductor is capable to provide a two-phase AC traveling magnetic field at high *** better understand the working principle,the present work improves a previous existing simplified theory by taking into account a complex electrical equivalent diagram due to the different mutual couplings between the two inductors and the two corresponding induced current sets.A more detailed theoretical model is provided,and the key and sensitive elements are *** on this theory,equipment is designed to provide a stirring effect on sodium chloride-salted water at 40 S/*** is shown that such a device fed by several hundred kiloHertz electric currents is able to mimic a linear motor.A set of optimized operating parameters are proposed to guide the experiment.A pure electromagnetic numerical model is *** modelling of the load is performed in order to assess the efficiency of the stirrer with a salt water *** a device can generate a significant liquid motion with both controlled flow patterns and adjus

关键词： Alternating magnetic field electromagnetic stirring low electrically conducting liquids ionic liquid

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An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis

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Plant Communications 2023年第5期4卷 277-294页

作者： Choghag Demirjian Narjes Razavi Gang Yu Baptiste Mayjonade Lu Zhang Fabien Lonjon Fabien Chardon Se bastien Carrere Je rome Gouzy Ste phane Genin Alberto PMacho Fabrice Roux Richard Berthome Fabienne Vailleau LIPME Universite de ToulouseINRAECNRSCastanet-TolosanFrance Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina universite Paris-Saclay INRAEAgroParisTechInstitut Jean-Pierre Bourgin(IJPB)78000 VersaillesFrance present address:Department of Cell&Systems Biology University of TorontoTorontoONCanada present address:Department of Biology NYU12 Waverly PlaceNew YorkNY 10003USA

Quantitative disease resistance(QDR)remains the most prevalent form of plant resistance in crop fields and wild ***-wide association studies(GWAS)have proved to be successful in deciphering the quantitative genetic basis of complex traits such as *** unravel the genetics of QDR to the devastating worldwide bacterial pathogen Ralstonia solanacearum,we performed a GWAS by challenging a highly polymorphic local mapping population of Arabidopsis thaliana with four *** type III effector(T3E)mutants,identified as key pathogenicity determinants after a first screen on an *** core collection of 25 *** most quantitative trait loci(QTLs)were highly specific to the identity of the T3E mutant(ripAC,ripAG,ripAQ,and ripU),we finely mapped a common QTL located on a cluster of nucleotide-binding domain and leucine-rich repeat(NLR)genes that exhibited structural *** functionally validated one of these NLRs as a susceptibility factor in response to ***,named it Bacterial Wilt Susceptibility 1(BWS1),and cloned two alleles that conferred contrasting levels of *** characterization indicated that expression of BWS1 leads to suppression of immunity triggered by different *** *** addition,we showed a direct interaction between BWS1 and RipAC T3E,and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1(SGT1b),the latter interaction being suppressed by ***,our results highlight a putative role for BWS1 as a quantitative susceptibility factor directly targeted by the T3E RipAC,mediating negative regulation of the SGT1-dependent immune response.

关键词： Ralstonia solanacearum typeⅢeffectors Arabidopsis thaliana GWAS R gene susceptibility

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Cellular and Molecular Requirements for Polar PIN Targeting and Transcytosis in Plants

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Molecular Plant 2008年第6期1卷 1056-1066页

作者： Jurgen Kleine-Vehn tukasz tangowski Justyna Wisniewska Pankaj Dhonukshe Philip B Brewer Jiri Friml Department of Plant Systems Biology VIB and Department of Molecular Genetics Ghent University 9052 Gent Belgium Zentrum fur Molekularbiologie der Pflanzen (ZMBP) Universitat Tubingen 72076 Tubingen Germany present address: Institute of General and Molecular Biology Nicolas Copernicus University 87-100 Torun Poland present address: Department of Biology Utrecht University 3508 TB Utrecht The Netherlands present address: Centre for Integrative Legume Research University of Queensland Brisbane Australia

The polar, sub-cellular localization of PIN auxin efflux carriers determines the direction of intercellular auxin flow, thus defining the spatial aspect of auxin signalling. Dynamic, transcytosis-like relocalizations of PIN proteins occur in response to external and internal signals, integrating these signals into changes in auxin distribution. Here, we examine the cellular and molecular mechanisms of polar PIN delivery and transcytosis. The mechanisms of the ARF-GEF-dependent polar targeting and transcytosis are well conserved and show little variations among diverse Arabidopsis ecotypes consistent with their fundamental importance in regulating plant development. At the cellular level, we refine previous findings on the role of the actin cytoskeleton in apical and basal PIN targeting, and identify a previously unknown role for microtubules, specifically in basal targeting. PIN protein delivery to different sides of the cell is mediated by ARFdependent trafficking with a previously unknown complex level of distinct ARF-GEF vesicle trafficking regulators. Our data suggest that alternative recruitment of PIN proteins by these distinct pathways can account for cell type- and cargo-specific aspects of polar targeting, as well as for polarity changes in response to different signals. The resulting dynamic PIN positioning to different sides of cells defines a three-dimensional pattern of auxin fluxes within plant tissues.

关键词： cytoskeleton polarity protein targeting.

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