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Effect of protease supplementation on apparent ileal crude protein and amino acid digestibility of over-processed soybean meals in broilers

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1810-1817页

作者： Sergio Salazar-Villanea Maikol Astúa-Ureña Allison Masís-Montoya Juan I.Herrera-Muñoz Catalina Salas-Durán Escuela de Zootecnia Universidad de Costa RicaSan JoséCosta Rica Centro de Investigación en nutrición Animal Universidad de Costa RicaSan JoséCosta Rica

Background: nutritional value of proteins in feed ingredients can be negatively affected by hydrothermal processing, which causes large variation in the bioavailability of amino acids(AA) and negatively affects animal productive performance. Supplementation of exogenous proteases could increase the rate of digestion of damaged proteins, thereby increasing overall AA digestibility and bioavailability. The aim was to determine the effect of exogenous protease supplementation on the apparent ileal digestibility(AID) of crude protein(CP) and AA of soybean meals(SBM) with different degrees of hydrothermal processing in ***: The experiment involved a 3 × 2 factorial arrangement, with SBM processing time(commercial SBM or autoclaved for 30 or 60 min at 120 ℃) and protease supplementation(not supplemented and supplemented) as factors. Protease was included at three times the recommended dose(0.06%) and the experimental diets were fed from 15 to 21 ***: The interaction between the effects of SBM processing and protease supplementation was significant for the AID of CP(P = 0.01), Trp(P = 0.01), Gly(P = 0.03) and Pro(P = 0.03), and also for the average daily gain(P = 0.01)and feed conversion ratio(P = 0.04). Increasing the processing time of SBM decreased(P no acids, whilst the effect of protease supplementation was only significant for the AID of Phe(P = 0.02) and Tyr(P = 0.01).Conclusions: Exogenous protease supplementation at three times the commercial dose does not seem to offset the negative effects of hydrothermal processing of SBM on the apparent ileal digestibility of CP and amino acids or performance of broilers. Whilst positive numerical improvements of digestibility and performance(ADG and FCR)were noticed with protease supplementation at relatively mild processing levels, negative results were obtained with the harsh-processed meals.

关键词： Amino acids Apparent ileal digestibility Broilers Exogenous protease

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novel Pectin/Chia-Mucilage Membranes:Human Serum Albumin Adsorption,Biocompatibility,and Physical-Chemical Properties

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Journal of Renewable Materials 2023年第6期11卷 2647-2663页

作者： S.E.Burruel-Ibarra R.A.Esquer-Osuna R.G.Valdez-Melchor D.A.Cuevas-Acuña L.Quihui-Cota J.Juárez J.C.Campos-García E.Valbuena-Gregorio M.A.López-Mata Departamento de Investigación en Polímeros y Materiales Universidad de SonoraHermosilloSonoraMéxico Departamento de Ciencias de la Salud Campus CajemeUniversidad de SonoraCd.ObregónSonoraMéxico Departamento de nutrición Pública y Salud Coordinación de NutriciónCentro de Investigación en Alimentación y DesarrolloA.C.HermosilloSonoraMéxico Departamento de Física Universidad de SonoraHermosilloSonoraMéxico

This study aimed to characterize the physical-chemical and biological properties of pectin(PC)/chia seed mucilage(CM)***/CM[100/0(control),80/20%,60/40%,and 40/60%w/w]membranes were prepared using the casting *** membranes(PC/CM)were thin,yellow,lightly opaque(≈10%)and capable of blocking light UVB(between 66 at 52%).SEM analysis showed the presence of aggregates in the shape of a sphere(≈13μm)and ovoid(≈25μm).The proportion of 80/20 showed an increase in tensile strength(29%)and elastic modulus(19%)when compared to the *** analysis exhibited intermolecular interactions between PCPC,PC-CM,and CM-CM in the *** thermal analysis(600°C)showed a slight improvement in the percentage of residual mass-loss of 3.31%(80/20)that *** 40/60 membrane showed the lowest percentage of hemolysis(2.94%)but limited human albumin adsorption *** results suggested that the blend PC/CM may be considered as a biomaterial for medical applications.

关键词： Pectin chia mucilage membrane hemolysis albumin biomaterial

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Caerulin-induced pancreatitis in rats: Histological and genetic expression changes from acute phase to recuperation

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World Journal of Gastroenterology 2006年第25期12卷 3999-4003页

作者： Javier Magaa-Gómez Guillermo López-Cervantes Ana María Calderón de la Barca Departamento de nutrición Centro de Investigación en Alimentación y Desarrollo A.C. HermosilloSonoraMéxicoDepartamento de Patología Hospital Infantil del Estado de SonoraHermosilloSonoraMéxicoDepartamento de Nutrición Centro de Investigación en Alimentación y DesarrolloA.C.HermosilloSonoraMéxico

AIM： To study the histological and pancreatitis-associated protein mRnA accumulation changes of pancreas from acute phase of caerulin-induced pancreatitis to recuperation in rats. METHODS： Acute pancreatitis was induced by caerulein in male Wistar rats and followed up for 90 d by histological and mRnA analyses of pancreas. Pancreases were dissected at 0, 9, 24 h and 3, 5, 15, 30, 60, 90 d post-induction. Edema （E）, polymorphonuclear neutrophU （pIVln） infiltration, cytoplasmic vacuolization （V）, zymogen granule depletion （ZD） and acinar disorganization （AD） were microscopically evaluated. Accumulation of pancreatitis- associated protein （PAP） and L13A mRnAs were quantified by real-time PCR. RESULTS： The main histological changes appeared at 9 h post-induction for PMn infiltration and cytoplasmic V, while at 24 h and 3 d for E and ZD, respectively. All the parameters were recovered after 5 d, except for ZD which delayed more than 30 d. The main AD was observed after 15 d and values returned to normal after 30 d. Similarly to histological changes, accumulation of the PAP mRnA was increased at 9 h with the highest accumulation at 24 h and differences disappeared after 5 d. COnCLUSIOn： From the acute phase to recuperation of pancreatitis, regeneration and re-differentiation of pan- creas occur and PAP expression is exclusively an acute response of pancreatitis.

关键词： Acute pancreatitis Histological changes PAP mRnA L13A mRnA

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Lanthanum-exchanged zeolite and clay as anticorrosive pigments for galvanized steel

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Journal of Rare Earths 2014年第4期32卷 352-359页

作者： S.Roselli n.Bellotti C.Deyá M.Revuelta B.del Amo R.Romagnoli CIDEPInT(Centro de Investigación y Desarrollo en Tecnología de Pinturas)(COnICET-CIC)

A wide variety of inhibitive pigments is now being offered as possible alternatives to chromate and lead compounds for painted metals protection. Unfortunately, the most wide spread of these substitute pigments, zinc phosphate, has, at present, raised some environmental concern because phosphate causes the eutrophication of water courses and zinc itself is toxic. The aim of this re-search was to study the anticorrosive performance of a mixture consisting of zinc phosphate, modified zeolite and clay （bentonite） in order to diminish phosphate content in paints. The zeolite and the clay were exchanged with La（III） ions, as inorganic green inhibitor. In the first step, the anticorrosion protection by La（III） ions in solution was assessed by electrochemical tests. In the second step, an epoxy-polyamide paint formulated with the pigment mixture applied on galvanized panels was studied by salt spray test and electro-chemical noise measurements （enM）. The results showed that it was possible to replace part of the zinc phosphate content in the paint with the exchanged zeolite and the clay.

关键词： exchanged pigments corrosion anticorrosive paints electrochemical noise measurements rare earths

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Historical fluxes of metal and metalloids in an aquatic ecosystem affected by land-use change and mining activities in northwestern Mexico

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International Journal of Sediment Research 2023年第5期38卷 724-738页

作者： Ochoa-Contreras Roberto Jara-Marini Martín enrique Ruiz-Fernández Ana Carolina Sanchez-Cabeza Joan Albert Meza-Figueroa Diana Pérez-Bernal Libia Hascibe Posgrado en Ciencias Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo 2. Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo Unidad Acad emica Mazatlán Instituto de Ciencias del Mar y Limnología Universidad Nacional Autónoma de M exico Departamento de Geología División de Ciencias Exactas y Naturales Universidad de Sonora Rosales y Encinas

Freshwater reservoirs are essential owing because of their ecological, economic, and social *** are particularly vulnerable to contamination, as of metal and metalloids, derived from anthropogenic activities like mining. The temporal variations in trace element concentrations(arsenic(As),mercury(Hg), chromium(Cr), copper(Cu), nickel(ni), lead(Pb), and zinc(Zn)), enrichment, fluxes, and possible sources were evaluated by studying two sediment cores from the La Angostura(AnG) Reservoir(northwest Mexico), using210Pb geochronology. The enrichment factors showed from null to minor enrichment for most elements, but moderate to severe enrichment of mercury(Hg). Most trace element concentrations had a detrital origin, and notable Hg concentration increases since the past decade were associated with severe drought periods, likely resulting from wildfires. The observed sediment concentrations of As and Hg can cause adverse effects on biota in the ecosystem since they are above the probable effect level(PEL). Development of strategies for metal attenuation in this reservoir is recommended and metals should be controlled until specific ecotoxicological studies are performed.

关键词： Aquatic ecosystems 210Pb geochronology Mining activities Metals Metalloids enrichment factors

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Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

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neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan Mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell Centro de Biología Molecular“Severo Ochoa”(CSIC-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain Department of Clinical neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden Departamento de Bioquímica y Biología Molecular Universidad Complutense de MadridMadridSpain Centro de Investigación Biomédica en Red de enfermedades neurodegenerativas(CIBERnED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

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Antioxidant Capacity and Bioaccessibility of Synergic Mango (cv. Ataulfo) Peel Phenolic Compounds in Edible Coatings Applied to Fresh-Cut Papaya

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Food and nutrition Sciences 2015年第3期6卷 365-373页

作者： Gustavo Rubén Velderrain-Rodríguez Maribel Ovando-Martínez Mónica Villegas-Ochoa Jesús Fernando Ayala-Zavala Abraham Wall-Medrano Emilio álvarez-Parrilla Tomás Jesús Madera-Santana Humberto Astiazarán-García Orlando Tortoledo-Ortiz Gustavo Adolfo González-Aguilar Coordinación en Tecnología de Alimentos de Origen Vegetal Centro de Investigación en Alimentación y Desarrollo Hermosillo México Instituto de Ciencias Biomédicas Universidad Autónoma de Ciudad Juárez Chihuahua México Departamento de nutrición Humana Coordinación de Nutrición Centro de Investigación en Alimentación y Desarrollo Hermosillo México

Edible coatings (EC) applied to fresh-cut fruits are used to increase their shelf-life and to deliver antioxidant bioactives such as phenolic compounds (PC) that reduce their oxidative damage while enhance their functional value. However, the combination of different PC may have synergetic, additive or antagonic effects on the final antioxidant capacity (AOXC). The aim of this study was to examine the AOXC of binary combinations of selected PC from mango peel and their bioaccessibility from 6% alginate-based EC applied to fresh-cut papaya, under simulated gastrointestinal conditions. Among equimolar (0.1 mM) combinations, gallic + protocatechuic acids (AB) were synergic in radical scavenging activity (RSA) as assayed by DPPH (90% RSA) and FRAP (0.39 mg TE/mL) methods;when assayed in 6% alginate-based EC, their RSA increased (117.85% RSA, 0.88 mg TE/mL). The application of EC + AB to papaya cubes and further in vitro digestion decreased their AOXC probably due to interactions between EC and papaya’s matrix. Therefore, further studies are needed in order to evaluate the effect of combination of phenolic and EC applied in other fruits matrix on antioxidants bioaccessibility.

关键词： Edible Coating Phenolic Acids Antioxidant Capacity Bioaccessibility

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Plumbing System of Hunga Tonga Hunga Ha'apai Volcano

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Journal of Earth Science 2023年第3期34卷 706-716页

作者： Román Alvarez Miguel Camacho Instituto de Investigaciones en Matemáticas Aplicadas y en Sistemas(IIMAS) Universidad Nacional Autónoma de MéxicoCoyoacán CDMX 04510Mexico Posgrado en Ciencias de la Tierra Universidad Nacional Autónoma de MéxicoCoyoacán CDMX 04510Mexico

The Hunga Tonga Hunga Ha’apai submarine volcano has experienced repeated eruptions in the latest *** recent one,in January 2022,released an enormous amount of energy inducing global perturbations,as tsunamis and atmospheric *** structure of the volcano is poorly understood,especially its internal ***-seated magmatic connections are difficult to define or *** use a high-resolution gravity data set obtained via satellite to calculate the Bouguer anomaly over its structure,to perform a preliminary exploration of its *** 3D gravity inversions,we find a complex plumbing system with various exhaust trajectories and multiple surface pockets of low-density material within the volcanic edifice;some appear to be associated with ring *** is in line with the report of the 2009 eruption,described as beginning from multiple *** found no signs of a magma chamber within 6 km depth,although several volcanic conduits are identified from such depth to the *** variations occur within a plumbing conduit or may vary from one conduit to another in the same *** models yield quantitative estimates for areas of magma-water interaction,constituting a baseline to compare with structural changes to be induced in future eruptions.

关键词： Hunga Tonga Hunga Ha'apai satellite gravity data 3D inversions volcano Tofua Island

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Effects of resveratrol and other polyphenols in hepatic steatosis

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World Journal of Gastroenterology 2014年第23期20卷 7366-7380页

作者： Leixuri Aguirre Maria Puy Portillo Elizabeth Hijona Luis Buja nutrition and Obesity Group Department of Nutrition and Food ScienceUniversity of Basque Country (UPV/EHU) and Centro de Investigación Lucio Lascaray01006 VitoriaSpain Centro de Investigación Biomédica en Fisiopatología de la Obesidad y nutrición (CIBERobn) Instituto de Salud Carlos Ⅲ01006 VitoriaSpain Department of Gastroenterology University of the Basque Country (UPV/EHU)Donostia Hospital and Biodonostia Institute20014 San SebastiánSpain Centro de Investigación Biomédica en enfermedades Hepáticas (CIBERehd) Instituto de Salud Carlos Ⅲ20014 San SebastianSpain

non-alcoholic fatty liver disease covers a wide spectrum of liver pathologies which range from simple steatosis to non-alcoholic *** are members of a very large family of plant-derived compounds that can have beneficial effects on human health,and thus their study has become an increasingly important area of human nutrition *** aim of the present review is to compile published data concerning the effects of both isolated polyphenols as well as polyphenol extracts,on hepatocyte and liver fat accumulation under different steatosis-inducing *** results reported clearly show that this group of biomolecules is able to reduce fat accumulation,but further studies are needed to establish the optimal dose and treatment period *** regard to the potential mechanisms of action,there is a good *** anti-lipidogenic effect of polyphenols is mainly due to reduced fatty acid and triacylglycerol synthesis,increased in fatty acid oxidation,and reduced of oxidative stress and *** a general conclusion,it can be stated that polyphenols are biomolecules which produce hepatoprotective *** date,these beneficial effects have been demonstrated in cultured cells and animal ***,studies performed in humans are needed before these molecules can be considered as truly useful tools in the prevention of liver steatosis.

关键词： Polyphenols Resveratrol Quercetin liver Steatosis non-alcoholic fatty liver disease

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Clinical Profiles at the Time of Diagnosis of SARS-CoV-2 Infection in Costa Rica During the Pre-vaccination Period Using a Machine Learning Approach

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Phenomics 2022年第5期2卷 312-322页

作者： Jose Arturo Molina-Mora Alejandra González Sergio Jiménez-Morgan Estela Cordero-Laurent Hebleen Brenes Claudio Soto-Garita Jorge Sequeira-Soto Francisco Duarte-Martínez Centro de Investigación en enfermedades Tropicales(CIET)and Facultad de Microbiología Universidad de Costa RicaSan José2060Costa Rica Instituto Costarricense de Investigación y enseñanza en nutrición y Salud(InCIenSA) Tres Ríos 30301Costa Rica Escuela de Medicina Universidad de Costa RicaSan José2060Costa Rica

The clinical manifestations of COVID-19,caused by the SARS-CoV-2,define a large spectrum of symptoms that are mainly dependent on the human host *** Costa Rica,more than 169,000 cases and 2185 deaths were reported during the year 2020,the pre-vaccination *** describe the clinical presentations at the time of diagnosis of SARS-CoV-2 infection in Costa Rica during the pre-vaccination period,we implemented a symptom-based clustering using machine learning to identify clusters or clinical profiles at the population level among 18,974 records of positive *** were compared based on symptoms,risk factors,viral load,and genomic features of the SARS-CoV-2 sequence.A total of 18 symptoms at time of diagnosis of SARS-CoV-2 infection were reported with a frequency>1%,and those were used to identify seven clinical profiles with a specific composition of clinical *** the comparison between clusters,a lower viral load was found for the asymptomatic group,while the risk factors and the SARS-CoV-2 genomic features were distributed among all the *** other distribution patterns were found for age,sex,vital status,and *** conclusion,during the pre-vaccination time in Costa Rica,the symptoms at the time of diagnosis of SARS-CoV-2 infection were described in clinical *** host co-morbidities and the SARS-CoV-2 genotypes are not specific of a particular profile,rather they are present in all the groups,including asymptomatic *** addition,this information can be used for decision-making by the local healthcare institutions(first point of contact with health professionals,case definition,or infrastructure).In further analyses,these results will be compared against the profiles of cases during the vaccination period.

关键词： COVID-19 Costa Rica Machine learning Diagnosis SARS-CoV-2 Clinical profiles

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