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Journal of Integrative Plant biology 2023年第5期65卷 1328-1343页

作者： Buwei Cao Linhao Ge Mingzhen Zhang Fangfang Li Xueping Zhou State Key Laboratory for biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China State Key Laboratory of Rice biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Autophagy is a conserved intracellular degradation process that plays an active role in plant response to virus *** we report that geminiviruses counteract activated autophagymediated antiviral defense in plant cells through the C2 proteins they *** found that,in Nicotiana benthamiana plants,tomato leaf curl Yunnan virus(TLCYnV)infection upregulated the transcription levels of autophagy-related genes(ATGs).Overexpression of NbATG5,NbATG7,or NbATG8a in *** plants decreased TLCYnV accumulation and attenuated viral ***,transgenic overexpression of NbATG7 promoted the growth of *** plants and enhanced plant resistance to *** further revealed that the C2 protein encoded by TLCYnV directly interacted with the ubiquitinactivating domain of *** interaction competitively disrupted the ATG7–ATG8 binding in *** and Solanum lycopersicum plants,thereby inhibiting autophagy ***,we uncovered that the C2-mediated autophagy inhibition mechanism was conserved in three other *** summary,we discovered a novel counter-defensive strategy employed by geminiviruses that enlists their C2 proteins as disrupters of ATG7–ATG8 interactions to defeat antiviral autophagy.

关键词： autophagy C2 counterdefense geminivirus plant defense the ATG7–ATG8 interaction

GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2013年第7期14卷 563-569页

作者： Li-li SUN Zhong-jing ZHOU Li-jun AN Yan AN Yong-qin ZHAO Xiao-fang MENG Clare STEELE-KING Yin-bo GAN Department of Agronomy College of Agriculture and Biotechnology Zhejiang University Centre for Novel agricultural Products Department of Biology University of York

Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first mor- phological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM.

关键词： Arabidopsis thaliana GLABROUS INFLORESCENCE STEMS (GIS) Endoreduplication SIAMESE (SIM) Trichome branching Genetic interaction

Role of rice cytosolic hexokinase OsHXK7 in sugar signaling and metabolism

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Journal of Integrative Plant biology 2016年第2期58卷 127-135页

作者： Hyun-Bi Kim Jung-Il Cho Nayeon Ryoo Dong-Ho Shin Youn-Il Park Yong-Sic Hwang Sang-Kyu Lee Gynheung An Jong-Seong Jeon Crop Biotech institute & Graduate School of biotechnology Kyung Hee University National Academy of agricultural Science Rural Development Administration Center for Neuroscience Korea Institute of Science and Technology Department of biology Chungnam National University Department of Bioscience and biotechnology Konkuk University

We characterized the function of the rice cytosolic hexokinase OsHXK7 （Qryza sativa HexokindseZ）, which is highly upregulated when seeds germinate under O2- deficient conditions. According to transient expression assays that used the promoter：luciferase fusion construct, OsHXK7 enhanced the glucose （Glc）-dependent repression of a rice n-amylase gene （RAmy3D） in the mesophyll protoplasts of maize, but its catalytically inactive mutant alleles did not. Consistently, the expression of OsHXK7, but not its catalytically inactive alleles, complemented the Arabidopsis glucose insensitive2-1 （gin2-1） mutant, thereby resulting in the wild type characteristics of Glc-dependent repression, seedling development, and plant growth. Interestingly, OsHXK7-mediated Glc-dependent repression was abolished in the O2-deficient mesophyll protoplasts of maize. This result provides compelling evidence that OsHXK7 functions in sugar signaling via a glycolysis-dependent manner under normal conditions, but its signaling role is suppressed when O2 is deficient. The germination of two null OsHXK7 mutants, oshxk7-1 and oshxk7-2, was affected by O2 deficiency, but overexpression enhanced germination in rice. This result suggests the distinct role that OsHXK7 plays in sugar metabolism and efficient germination by enforcing glycolysis-mediated fermentation in O2-deficient rice.

关键词： Germination metabolism OsHXK7 O2 deficiency rice signaling sugar

Development and Identification of Novel Rice Blast Resistant Sources and Their Characterization Using Molecular Markers

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Rice science 2015年第6期22卷 300-308页

作者： S.J.S.RAMA DEVI Kuldeep SINGH B.UMAKANTH B.VISHALAKSHI P.RENUKA K.VIJAY SUDHAKAR M.S.PRASAD B.C.VIRAKTAMATH V.RAVINDRA BABU M.S.MADHAV biotechnology Crop ImprovementIndian Institute of Rice ResearchIndian Council of Agriculture Research School of agricultural biotechnology Punjab Agricultural University Plant Pathology Indian Institute of Rice ResearchIndian Council of Agriculture Research

To develop and characterize introgression lines for leaf and neck blast resistance, 326 introgression lines were developed using various accessions of six different AA genome wild species in the genetic background of elite Indian varieties like PRl14 and Pusa 44 and were screened for blast resistance. Stringent phenotyping coupled with genotyping using gene based markers led to the identification of four resistant introgression lines, which showed promising resistance and do not possess any of the tested genes. Furthermore, multi-location screening confirmed the field resistance of the four introgression lines to both leaf and neck blast. Molecular characterization of these introgression lines using genome-wide simple sequence repeat markers revealed the presence of small percentage of wild Oryza genome introgrssion. So these lines can be used for mapping and identification of novel leaf and neck blast resistance genes. Thus, these four introgression lines can be considered as new genetic resources for blast resistance.

关键词： rice blast resistance introgression line gene profiling wild species

The Origin of Alligator sinensis

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Science China Chemistry 1993年第3期36卷 279-287页

作者：莫鑫泉赵铁军秦鹏春 Molecular Evolution Laboratory Institute of Developmental Biology Academia Sinica Beijing 100080 PRC Department of biotechnology Northeast Agricultural College Harbin 150030 PRC

Alligator sinensis and Alligator mississippiensis are put under the Alligator family and Alligator genus as two different species and have been thought that they are closely related in taxonomy. The results of our DNA-DNA hybridization experiments showed that A. sinensis is a relatively young species originated in Asia,whereas A. mississippiensis is the *** seems that the alligator and crocodilian could evolve from each *** evolutionary rate of the crocodilian is slower than those of birds and higher *** phylogeny of four crocodilians have been obtained and the hypothesis that the ancestor of Crocodylus porosus is a transitional form between *** and *** is proposed.

关键词： Yangzi Alligator phylogeny DNA-DNA hybridization.

LOCATION OF 43 kD MOSQUITO-LARVICIDAL TOXIN GENE OF HIGHLY TOXIC Bacillus sphaericus 10

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Science China Chemistry 1991年第5期34卷 593-598页

作者：刘阳范云六 Lab of Molecular biology Biotechnology Research Center Chinese Academy of Agricultural Sciences Beijing 100081 PRC

The direct evidence of the location of the mosquito-larvicidal gene of Bacillus sphaericus10 (isolated from Jiangsu Province of China) on megaplasmid pFW1 was given by molecularcloning. The clone (pFL109) containing the 1.4 kb HindⅢ DNA fragment from pFW1expressed the mosquito-larvicidal toxin protein (43 kD). The location of the gene codingfor the 43 kD toxin protein within the Xhol B fragment on the restriction map of pFW1 wasconfirmed by Southern blotting using the 1.4 kb HindⅢ DNA fragment as a probe. The non-toxic strains of Bacillus sphaericus were revealed to be 43 kD toxin gene dele-tion mutants by Southern and Western analyses. The 1.4 kb HindⅢ fragment of pFL109 can be used as a probe for differentiating thenon-toxic strains of Bacillus sphaericus from the toxic ones.

关键词： Bacillus sphaericus BS megaplasmid pFW1 larvicidal toxin gene gene location

Potato tonoplast sugar transporter 1 controls tuber sugar accumulation during postharvest cold storage

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Horticulture Research 2023年第4期10卷 200-210页

作者： Tengfei Liu Md Abu Kawochar Shahnewaz Begum Enshuang Wang Tingting Zhou Shenglin Jing Tiantian Liu Liu Yu Bihua Nie Botao Song Key Laboratory of Horticultural Plant biology Ministry of EducationKey Laboratory of Potato Biology and BiotechnologyMinistry of Agriculture and Rural AffairsCollege of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070 China Bangladesh agricultural Research institute JoydebpurGazipur 1701Bangladesh

Cold-induced sweetening(CIS),the undesirable sugar accumulation in cold-stored potato(Solanum tuberosum L.)tubers,is a severe postharvest issue in the potato processing *** the process of sucrose hydrolysis by vacuolar invertase during potato CIS is well understood,there is limited knowledge about the transportation of sucrose from the cytosol to the vacuole during postharvest cold ***,we report that among the three potato tonoplast sugar transporters(TSTs),StTST1 exhibits the highest expression in tubers during postharvest cold *** localization analysis demonstrates that StTST1 is a tonoplast-localized ***1 knockdown decreases reducing sugar accumulation in tubers during low-temperature *** to wild-type,potato chips produced from StTST1-silenced tubers displayed significantly lower acrylamide levels and lighter color after cold *** analysis manifests that suppression of StTST1 promotes starch synthesis and inhibits starch degradation in cold-stored *** further establish that the increased sucrose content in the StTST1-silenced tubers might cause a decrease in the ABA content,thereby inhibiting the ABA-signaling *** demonstrate that the down-regulation ofβ-amylase StBAM1 in StTST1-silenced tubers might be directly controlled by ABA-responsive element-binding proteins(AREBs).Altogether,we have shown that StTST1 plays a critical role in sugar accumulation and starchmetabolism regulation during postharvest cold ***,our findings provide a newstrategy to improve the frying quality of cold-stored tubers and reduce the acrylamide content in potato chips.

关键词： sugar acrylamide hydrolysis

Transgenic Tobacco Plants by Cotransformation With Proteinase Inhibitor Ⅱ and δ-endotoxin Genes

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Chinese Science Bulletin 1993年第18期38卷 1561-1566页

作者：华学军陈晓邦马万超范云六 Molecular biology Laboratory Biotechnology Research Centre Chinese Academy of Agricultural Sciences Beijing 100080 PRC

In recent years, great progress has been made in. producing plants resistant to insect attack by genetic engineering of B. t δ-endotoxin gene or plant proteinase inhibitor genes, and how to avoid the emergence of the insect resistance to B. t δ-endotoxin produced by transgenic plants has been paid attention to. To combine one B. t δ-endotoxin gene with an incompatible B. t δ-endotoxin gene or with other genes such as proteinase inhibitor gene might alleviate the insect resistance problem. In this note, we study the

关键词： proteinase inhibitor gene B. t δ-endotoxin gene cotransformation transgenic plants gene expression.

Ipomoea batatas HKT1 transporter homolog mediates K^+ and Na^+ uptake in Saccharomyces cerevisiae

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Journal of Integrative Agriculture 2017年第10期16卷 2168-2176页

作者： PARK Sung-chul YU Yi-cheng KOU Meng YAN Hui TANG Wei WANG Xin LIU Ya-ju ZHANG Yun-gang KWAK Sang-soo MA Dai-fu SUN Jian LI Qiang Xuzhou institute of agricultural Sciences in Jiangsu Xuhuai District/Key Laboratory for biology and Genetic Breeding of Sweet Potato Ministry of Agriculture/Sweet Potato Research InstituteChinese Academy of Agricultural Science institute of Integrative Plant biology School of Life ScienceJiangsu Normal University Plant Systems Engineering Research Center Korea Research Institute of Bioscience and Biotechnology

Soil salinity causes the negative effects on the growth and yield of crops. In this study, two sweet potato （Ipomoea batatas L.） cultivars, Xushu 28 （X-28） and Okinawa 100 （O-100）, were examined under 50 and 100 mmol L-1 NaCI stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly, real-time quantitative polymerase chain reaction （RT-qPCR） results indicated that sweet potato high-affinity K^＋ transporter 1 （IbHKT1） gene expression was highly induced by 50 and 100 mmol L-1 NaCI stress in the stems of X-28 cultivar than in those of O-100 cultivar, but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter, we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium （Na^＋）. Yeast complementation assay showed that IbHKT1 could take up K^＋ in 50 mmol L^-1 K^＋ medium without the presence of NaCI. Moreover, Na^＋ uptake significantly increased in yeast overexpressing IbHKTI. These results showed that IbHKT1 transporter could have K^＋-Na^＋ symport function in yeast. Therefore, the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other HKT1 transporters in class I. Potentially, IbHKT1 could be used to improve the salt tolerance nature in sweet potato.

关键词： IbHKT1 Na^+/K^+transporter salt stress sweet potato

Expression of two insect-resistant genes cryIA (b&c)/GNA in transgenic tobacco plants results in added protection against both cotton bollworm and aphids

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Chinese Science Bulletin 1999年第22期44卷 2051-2058页

作者： WANG Zhibin and QUO SanduiLaboratory of Biochemistry and Molecular biology , biotechnology Research Center, Chinese Academy of agricultural Sciences , Beijing 100081, China Laboratory of Biochemistry and Molecular biology Biotechnology Research Center Chinese Academy of Agricultural Sciences Beijing China

The synthesized Bacillus thuringiensis insecticidal protein gene crylA(b&c) and the synthesized gene GNA, (the mannose specific lectin from snowdrop ( Galanthus nivalis)), tumefaciens have been inserted into plant expression vector pGW4BAI. Leave stripes of Nico-tiana tabacum var. K326 have been transformed with Agrobacterium tumefaciens strain LBA4404 harboring the plant expression vector. 28 kanamycin resistant tobacco plants

关键词： crylA(b&c) snowdrop lectin insect-resistance transgenic plants cotton bollworm aphids pyramiding.